目的 采用多指标成分测定结合化学计量学评价石菖蒲的质量，并与伪品水菖蒲、金钱菖蒲进行鉴别。方法 采用GC法测定石菖蒲中草蒿脑、甲基丁香酚、异丁香酚甲醚、γ-细辛醚、β-细辛醚、α-细辛醚6个成分，并结合偏最小二乘判别分析、正交偏最小二乘判别分析、层次聚类分析评价石菖蒲，与其伪品水菖蒲、金钱菖蒲进行鉴别。结果 石菖蒲、水菖蒲、金钱菖蒲分别聚为3类。草蒿脑、β-细辛醚可以作为石菖蒲区别于常见伪品水菖蒲、金钱菖蒲的差异特征标志物。结论 本方法简便、快速、稳定，同时定量6个成分，可用于石菖蒲与常见伪品水菖蒲、金钱菖蒲的区分。

Objective To evaluate the quality of Acorus tatarinowii by multi-index component analysis combined with chemometrics, and to identify it from the common counterfeit products A. calamus and A. gramineus. Methods A GC method was established to determine the contents of estragole, methyl eugenol, isoeugenol methyl ether, γ-asarone, β-asarone, and α-asarone in A. tatarinowii. The method was combined with partial least squares discriminant analysis, orthogonal partial least squares discriminant analysis, and hierarchical cluster analysis to identify A. tatarinowii from its counterfeit products A. calamus and A. gramineus. Results A. tatarinowii, A. calamus, and A. gramineu were respectively classified into three categories. Estragole and β-asarone could be used as differential characteristic markers to distinguish A. tatarinowii from common counterfeit products A. calamus and A. gramineus. Conclusion This method is simple, rapid, and stable, and can quantify six components simultaneously. It can be used to distinguish A. tatarinowii from its counterfeit products A. calamus and A. gramineus.

R286.02

工业信息化部产业技术基础公共服务平台项目(2022-230-221);佛山市南海区重点领域科技攻关专项(南科[2023]20号-18)