目的 建立仙茅HPLC多指标成分定量-一测多评法，采用化学计量学、加权TOPSIS和灰色关联度（GRA）融合模型评价不同产地仙茅质量。方法 采用HPLC-一测多评法测定不同产地仙茅中仙茅苷乙、苔黑酚龙胆二糖苷、苔黑酚葡萄糖苷、苔黑素、2,6-二甲氧基苯甲酸、仙茅苷、仙茅木酚素、豆甾醇、胡萝卜苷、β-谷甾醇，并检测浸出物、总灰分和酸不溶性灰分，采用化学计量学分析数据，建立加权TOPSIS和GRA融合模型评价仙茅药材质量。结果 不同产地仙茅中各成分含量稍有波动；仙茅样品大致分为3类；苔黑酚葡萄糖苷、仙茅苷、仙茅苷乙、苔黑酚龙胆二糖苷、仙茅木酚素和β-谷甾醇可作为不同产地仙茅的质量差异因子。不同产地仙茅质量存在显著差异，其中编号S14的仙茅样品的质量最优。结论 建立的方法简单、检测周期短，利于推广，能较全面地评价仙茅的质量。

Objective To establish an HPLC multi-index component analysis - QAMS method, and to evaluate the quality of Curculiginis Rhizoma from different regions by chemometrics, weighted TOPSIS, and GRA fusion model. Methods The contents of curculigoside B, orcinol gentiobioside, orcinol glucoside, orcinol, 2,6-dimethoxybenzoic acid, curculigoside, curlignan, stigmasterol, daucosterol, and β-sitosterol in Curculiginis Rhizoma were determined by HPLC - QAMS method, and the extract, total ash, and acid-insoluble ash were also detected. Data were analyzed by chemometrics and the weighted TOPSIS and GRA fusion model was established to evaluate the quality of Curculiginis Rhizoma. Results The content of various components in Curculiginis Rhizoma from different origins fluctuated slightly. Curculiginis Rhizoma were divided into 3 groups. Orcinol glucoside, curculigoside, curculigoside B, orcinol gentiobioside, curlignan and β-sitosterol could be used as quality differential factors for different origins of Curculiginis Rhizoma. There were significant differences in the quality of Curculiginis Rhizoma from different regions, among which sample S14 had the best quality. Conclusion The established method is simple with short measurement time, which is conducive to promotion and can comprehensively evaluate the quality of Curculiginis Rhizoma.

R286.02

河南省高等学校重点科研资助计划项目(20A110024)