目的 利用网络药理学及分子对接技术探索快胃片治疗慢性非萎缩性胃炎的作用机制。方法 利用中药系统药理学数据库与分析平台（TCMSP）和HERB数据库获取快胃片的活性成分及其靶点，从GeneCards、OMIM数据库中获取慢性非萎缩性胃炎的疾病靶点，取快胃片活性成分及慢性非萎缩性胃炎的交集靶点。通过STRING数据库对交集靶点进行蛋白质互作分析，Metascape对交集靶点进行基因本体（GO）与京都基因与基因组百科全书（KEGG）富集分析。利用AutodockTools对快胃片的关键成分及核心靶点进行分子对接初步验证。结果 网络药理学研究共获取152个快胃片有效活性成分，包括槲皮素、山柰酚、糖蛋白、芒柄花素等，385个成分靶点，1 082个慢性非萎缩性胃炎靶点，取交集获得共同靶点112个，其中核心靶点为肿瘤蛋白p53（TP53）、肿瘤坏死因子（TNF）、蛋白激酶B1（Akt1）、JUN、白细胞介素-1β（IL-1β）。GO富集分析显示生物过程（BP）有1 650条，主要包括积极调节细胞迁移、对细菌源性分子的反应、细胞程序性死亡的正向调节；生物功能（MF）有141条，主要包括蛋白激酶结合、激酶调节器活性、细胞因子受体结合；细胞组成（CC）有66条，主要包细胞外基质、膜筏和分泌颗粒腔。KEGG富集分析得到通路188条，主要包括癌症的发病途径、流体剪切应力与动脉粥样硬化、IL-17信号通路、癌症中的蛋白聚糖、化学致癌-活性氧等通路。分子对接结果显示快胃片有效成分与核心靶点能够稳定结合。结论 快胃片可通过多成分、多靶点、多通路来对慢性非萎缩性胃炎发挥作用，防止其向慢性萎缩性胃炎转变。

Objective To study the mechanism of Kuaiwei Tablets in treating chronic non-atrophic gastritis based on the methods of network pharmacology and molecular docking technology. Methods The TCMSP database and HERB database were used to obtain the active ingredients and targets of Kuaiwei Tablets, and the disease targets of chronic non-atrophic gastritis were obtained from GeneCards and OMIM databases, and then took the common targets of Kuaiwei Tablets and chronic non-atrophic gastritis. PPI network of the common targets was analysed by STRING database, and GO and KEGG pathway enrichment were analysed by Metascape. AutodockTools was used for molecular docking of the key components and targets of Kuaiwei Tablets. Results A total of 152 active ingredients such as quercetin, kaempferol, glycoprotein, formononetin and so on, 385 ingredient targets, 1 082 targets of chronic non-atrophic gastritis and 112 common targets were obtained from the network pharmacological study. The core targets were TP53, TNF, Akt1, JUN, IL-1β, etc. GO analysis showed that BP (1 650 pieces) mainly involve positive regulation of cell migration, response to molecule of bacterial origin, and positive regulation of programmed cell death, etc. MF (141 pieces) mainly involved kinase binding, kinase regulator activity, and cytokine receptor binding, etc. CC (66 pieces) mainly involved extracellular matrix, membrane raft, and secretory granule lumen, etc. There were 188 pathways obtained in the KEGG enrichment analysis, mainly including pathways in cancer, fluid shear stress and atherosclerosis, IL-17 signaling pathway, proteoglycans in cancer, chemical carcinogenesis-reactive oxygen species, etc. The molecular docking results showed that the effective components of Kuaiwei Tablets and the core targets could combined stably. Conclusion Kuaiwei Tablets can work on CNAG through multi-components, multi-targets and multi-pathways to prevent its transformation to chronic atrophic gastritis.

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齐鲁医派中医学术流派传承项目;齐鲁伤寒六经辨证中医药特色技术