目的 建立UPLC法测定小柴胡颗粒中柴胡皂苷b₁、b₂。方法 使用Acquity UPLC BHE C₁₈色谱柱（100 mm×2.1 mm，1.7μm）；流动相：水–乙腈，梯度洗脱；检测波长：254 nm；体积流量：0.3 mL/min；柱温：30 ℃；进样量3 μL。结果 柴胡皂苷b₁、b₂线性范围分别为0.006～0.374 mg、0.003～0.214 mg，平均回收率分别为98.97%、99.35%，RSD值分别为1.82%、1.16%。结论 所建立方法准确、稳定，重现性好，可用于小柴胡颗粒的质量控制和评价。

Objective To develop an UPLC method for determination the saikosaponin b₁ and saikosaponin b₂ in Xiaochaihu Granules .Methods The analysis was applied on Acquity UPLC BHE C₁₈ column (100 mm × 2.1 mm, 1.7 μm). The mobile phase consisted of water and acetonitrile with gradient elution. The detection wavelength was set at 254 nm, flow rate was 0.3 mL/min, column temperature was 30 ℃, and volume of injection was 3 μL. Results Saikosaponin b₁ and saikosaponin b₂ showed good linear relationships of 0.006 — 0.374 mg and 0.003 — 0.214 mg, whose average recoveries were 98.97% and 99.35% with RSD values of 1.82% and 1.16%. Conclusion The established method is accurate, stable, and has good reproducibility, and can be used for quality control and evaluation for Xiaochaihu Granules.

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北京市中医药管理局第三批中药骨干人才培养项目（京中医科字[2022]59号）