目的 探究蓝萼乙素通过蛋白激酶B（Akt）/B细胞淋巴瘤-2（Bcl-2）相关死亡启动因子（BAD）通路对宫颈癌裸鼠移植瘤生长的影响和机制。方法 将48只SPF级雌性Balb/c-nu裸鼠随机分为模型、蓝萼乙素（56、112 mg/kg）和蓝萼乙素＋SC79（112 mg/kg＋10 mg/kg）组，每组12只。将HeLa细胞种植在裸鼠右大腿根部背侧皮下，建立皮下移植瘤模型，观察肿瘤体积、质量和一般情况的变化。苏木精–伊红（HE）染色观察肿瘤组织的组织学变化。TUNEL实验检测肿瘤组织细胞凋亡情况。Western blotting法检测移植瘤组织中p-Akt、Akt、p-BAD、BAD、磷酸酯酶与张力蛋白同源物（PTEN）蛋白表达水平。结果 与模型组相比，蓝萼乙素组第22、25、28天裸鼠肿瘤体积、肿瘤质量、肿瘤组织Akt、BAD磷酸化水平显著降低（P＜0.05），肿瘤细胞凋亡率、肿瘤组织PTEN蛋白水平显著升高（P＜0.05）；SC79可减弱蓝萼乙素对宫颈癌裸鼠移植瘤生长的抑制作用。结论 蓝萼乙素对宫颈癌裸鼠移植瘤生长起到抑制作用，其作用机制与抑制Akt/BAD信号通路有关。

Objective To investigate the impact and mechanism of glaucocalyxin B on the growth of cervical cancer transplanted tumor in nude mice through the Akt/Bcl-2 associated BAD pathway. Methods 48 SPF grade female Balb/c nu nude mice were randomly grouped into model group, glaucocalyxin B (56, 112 mg/kg), and glaucocalyxin B + SC79 (112 mg/kg + 10 mg/kg) group, with 12 mice in each group. HeLa cells were implanted subcutaneously at the dorsal side of the right thigh root in nude mice to establish a subcutaneous transplanted tumor model, and the changes in tumor volume, weight, and general situation were observed. HE staining was applied to observe the pathological changes of tumor tissue. TUNEL assay was applied to detect the apoptosis of tumor histiocyte. Western blotting method was applied to detect the expression levels of p-Akt, Akt, p-BAD, BAD, and PTEN proteins in transplanted tumor tissue. Results Compared with the model group, the tumor volume at 22, 25, and 28 day, tumor weight, Akt, and BAD phosphorylation levels in tumor tissue of the glaucocalyxin B group were obviously decreased (P < 0.05), the apoptosis rate of tumor cells and the expression of PTEN protein in tumor tissue were obviously increased (P < 0.05). SC79 could weaken the inhibitory effect of glaucocalyxin B on the growth of cervical cancer transplanted tumor in nude mice. Conclusion Glaucocalyxin B inhibited the growth of transplanted tumors in nude mice with cervical cancer, and its mechanism of action was related to the inhibition of the Akt/BAD signaling pathway.

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开封市科技发展计划项目（2103067）