目的 评价不同产地肺形草的质量差异。方法 采用HPLC法测定肺形草中马钱苷酸、莫诺苷、獐牙菜苦苷、龙胆苦苷、芒果苷、异荭草苷、异牡荆素、去甲基雏菊叶龙胆酮、当药黄素、3,6,8-三羟基-1-甲基呫吨酮、山楂酸和熊果酸，对多指标成分测定结果进行主成分分析、正交偏最小二乘法-判别分析，建立加权优劣解距离模型评估。结果 16批肺形草明显聚集为3类；马钱苷酸、莫诺苷、3,6,8-三羟基-1-甲基呫吨酮、熊果酸、当药黄素和芒果苷是影响肺形草质量的主要潜在标志物；浙江和江苏产肺形草整体质量较好。结论 建立的方法控制模式简单、评价模型科学合理，可用于肺形草质量评价。

Objective To evaluate the quality difference of Tripterospermum chinense from different origins. Methods Contents of loganic acid, morroniside, swertiamain, gentiopicrin, mangiferin, isoorientin, isovitexin, demethylbellidifolin, swertisin, 3,6,8-trihydroxy-1-methyl-xanthen-9-one, maslinic acid and ursolic acid in T. chinense were determined by HPLC method. PCA and OPLS-DA were used to analyze the contents of multi-component components, and the weighted-TOPSIS model was established to evaluate the quality. Results 16 Batches of T. chinense were clustered into three categories, and loganic acid, morroniside, 3,6,8-trihydroxy-1-methyl-xanthen-9-one, ursolic acid, swertisin, and mangiferin were the main potential markers affecting the quality of T. chinense. The overall quality of T. chinense from Zhejiang and Jiangsu Provinces is good. Conclusion The established method has a simple control mode and a scientifically reasonable evaluation model, which can be used for the quality evaluation of T. chinense.

R286.02

上海市“医苑新星”青年医学人才培养资助计划项目（沪卫人事[2022]65号）