目的 基于血清药物化学技术对五酯胶囊中成分和大鼠入血成分进行鉴定。方法 采用UPLC-Q-TOF-MS法对五酯胶囊成分组、给药组和空白组样品进行分析，通过MassLynx V4.1软件对所得图谱进行数据解析，对五酯胶囊中成分及其入血成分进行鉴定。结果 鉴定出五酯胶囊中15个化合物，其中五味子甲素含量最高。大鼠血清中含有五酯胶囊2个原型成分五味子酯丁、长南酸，筛选得到代谢物8个，主要以I相代谢反应中的水解、氧化、去甲氧基化反应和II相代谢反应中的葡萄糖醛酸化结合为主。结论 建立了五酯胶囊中活性成分和代谢产物的鉴定方法，为五酯胶囊的临床应用提供依据。

Objective To identify the components in Wuzhi Capsules and rats blood components based on serum pharmacochemistry technology. Methods Samples of ingredients of Wuzhi Capsules group, Wuzhi Capsules group, and blank group were analyzed by UPLC-Q-TOF-MS method. The data were analyzed by MassLynx V4.1 software, and the components in Wuzhi Capsules and rats blood components were identified. Results There were 15 identified components in in Wuzhi Capsules, and the content of schisandrin A was the highest. The serum of rats contained two prototype components in Wushizi Capsules, namely schisantherin D and changnanic acid, and 8 metabolites were screened out. These components were mainly composed of hydrolysis, oxidation, and demethoxylation reaction in phase I metabolic reactions, as well as glucuronidation in phase II metabolic reaction. Conclusion The method can identify active ingredients and metabolites in Wuzhi Capsules, providing a basis for clinical application of Wushizi Capsules.

R286.02

国家自然科学基金资助项目（81903938）