目的 建立指纹图谱和多指标成分测定方法评价不同产地广东王不留行药材。方法 采用UPLC法建立广东王不留行药材特征图谱和绿原酸、芦丁的测定方法,采用熵权法所得权重作为分辨系数构建广东王不留行的质量评价模型,灰色关联度法计算各指标的相关关联度。结果 广东王不留行UPLC特征图谱共确定8个共有峰,指认了其中3个成分新绿原酸、绿原酸、隐绿原酸。测定了13批不同产地广东王不留行中绿原酸和芦丁。13批广东王不留行药材的相对关联度范围为0.116 2~0.810 5,不同批次样品之间的质量存在一定的差异,广东肇庆产广东王不留行的质量较优。结论 建立的方法可有效评价不同产地广东王不留行的质量,为广东王不留行的药材质量控制提供参考。

Objective To evaluate the crude drug of Fici Pumilae Receptaculum from different origins by characteristic chromatogram and multiple index. Methods UPLC was used to establish the characteristic chromatogram and the determination of chlorogenic acid and rutin in Fici Pumilae Receptaculum, and the quality evaluation model of Fici Pumilae Receptaculum was established by using the entropy weight method as the resolution coefficient. The correlation between various indicators was calculated by grey relation analysis. Results Eight common peaks were established in UPLC characteristic chromatogram, and that 3 of them were identified as neochlorogenic acid, chlorogenic acid, and cryptochlorogenic acid. Contents of chlorogenic acid and rutin in 13 batches of Fici Pumilae Receptaculum from different origins were determined. The relative correlation degree of 13 batches of samples ranged from 0.116 2 to 0.810 5. There were certain differences in quality between different batches of samples, and Fici Pumilae Receptaculum samples from Zhaoqing City in Guangdong Province was better. Conclusion The method can effectively evaluate the quality of Fici Pumilae Receptaculum from different origins, providing reference for the quality control of Fici Pumilae Receptaculum crude drug.

R286.02

国家工信部产业技术基础公共服务平台项目（2022-230-221）；佛山市南海区重点领域科技攻关专项（南科[2023]20号-18）