目的 研究植物乳杆菌RG-034的体外抑菌效果以及对大鼠腹泻型肠易激综合征的治疗作用。方法 采用活菌计数法测定RG-034菌粉活菌数,利用类"牛津杯法"确定植物乳杆菌RG-034对大肠埃希菌、乙型副伤寒沙门菌、金黄色葡萄球菌的抑菌活性。SD大鼠按体质量随机分为对照组、模型组、蒙脱石散组及植物乳杆菌RG-034胶囊组,每组6只。除对照组外,其余大鼠每日ig 5 g/kg番泻叶联合隔日禁食,持续5周,制备大鼠腹泻型肠易激综合征模型。自造模第3周开始给药,植物乳杆菌RG-034组大鼠ig RG-034胶囊(1×10⁹ CFU/粒,15 mg),2粒/d;蒙脱石散组大鼠ig 0.81 g/kg蒙脱石散,1次/d;对照组和模型组大鼠ig等量生理盐水,给药3周。观察大鼠一般状态变化,测定稀便率、稀便级以及腹泻指数,采用16S核糖体RNA测序技术分析大鼠结肠中肠道菌群变化。结果 植物乳杆菌RG-034菌粉活菌数为2.5×10¹¹ CFU/g,对不同浓度的大肠埃希菌、乙型副伤寒沙门菌、金黄色葡萄球菌均有一定的抑制作用,抑菌圈直径为15.90~25.06 mm。与模型组比较,植物乳杆菌RG-034胶囊可明显改善大鼠腹泻情况,显著降低稀便率、稀便级以及腹泻指数,增加了肠道菌群多样性。结论 植物乳杆菌RG-034对大鼠腹泻型肠易激综合征具有一定的治疗作用,其机制可能与调节肠道菌群多样性有关。

Objective To investigate the bacteriostatic effect of Lactobacillus plantarum RG-034 in vitro and its therapeutic effect on the diarrhoea-predominant irritable bowel syndrome in rats. Methods The viable bacteria count of RG-034 powder was determined by using the "Oxford Cup method" to determine the antibacterial activity of Lactobacillus plantarum RG-034 against Escherichia coli, Salmonella paratyphi B and Staphylococcus aureus. SD rats were randomly divided into control group, model group, Montmorillonite Powder group, and Lactobacillus plantarum RG-034 capsule group by body weight, with 6 rats in each group. Except the control group, other rats were given 5 g/kg senna leaf combined with alternate day fasting daily for 5 weeks to prepare a rat model of diarrhea-type irritable bowel syndrome. At the 3rd week of self-modeling, rats in Lactobacillus plantarum RG-034 group were given RG-034 Capsules (1 × 10⁹ CFU/Capsule, 15 mg), 2 capsules/d. Rats in Montmorillonite Powder group were ig 0.81 g/kg Montmorillonite Powder once daily. The rats in control group and model group were given the same amount of normal saline intragaically for 3 weeks. The changes of the general status of rats were observed, the rate of loose stool, the grade of loose stool and the diarrhea index were determined, and the changes of intestinal flora in the colon of rats were analyzed by 16S ribosomal RNA sequencing technology. Results The viable bacteria number of Lactobacillus plantarum RG-034 Powder was 2.5 × 10¹¹ CFU/g, which had certain inhibitory effect on Escherichia coli, Salmonella paratyphi B and Staphylococcus aureus at different concentrations, and the inhibitory zone diameter was 15.90-25.06 mm. Compared with the model group, Lactobacillus plantarum RG-034 Capsules can significantly improve the diarrhea situation of rats, significantly reduce the rate of loose stool, loose stool grade and diarrhea index, and increase the diversity of intestinal flora. Conclusion Lactobacillus plantarum RG-034 has a certain therapeutic effect on diarrhea-predominant irritable bowel syndrome in rats, and its mechanism may be related to the regulation of intestinal microbiota diversity.

R965