目的 建立茜草多指标成分定量联合化学计量学评价不同产地茜草的质量。方法 采用高效液相色谱法测定8省区18批茜草中新橙皮苷、山柰酚、茜草素、异茜草素、羟基茜草素、1,3,6-三羟基-2-甲基蒽醌、大叶茜草素、去氢-α-拉杷醌、β-谷甾醇和异落叶松脂素，并采用主成分分析、正交偏最小二乘法-判别分析、灰色关联度分析对测定结果进行评价。结果 相同产区的茜草，质量较接近；共找到4个差异性标志成分，其影响显著性排序为大叶茜草素＞茜草素＞1,3,6-三羟基-2-甲基蒽醌＞羟基茜草素；陕西、河南和安徽所产的茜草整体质量较好。结论 方法便捷、准确，易于推广，可以更全面、更系统地评价茜草的质量。

Objective To establish a method for quality evaluation of Rubiae Radix by HPLC combined with chemometrics. Methods The contents of neohesperidin, kaempferol, alizarin, xanthopurpurin, purpurin, 1,3,6-trihydroxy-2-methylanthraquinone, mollugin, dehydro-α-lapachone, β-sitosterol, and isolariciresinol in 18 batches of Rubiae Radix collected from 8 provinces were determined by HPLC method, and the results were analyzed by principal component analysis (PCA), orthogonal partial least squares discriminant analysis (OPLS-DA), and grey relational analysis (GRA) method. Results Rubiae Radix from the same production area had similar quality. A total of four differential marker components were found, and their significance was ranked as follows: mollugin ＞ alizarin ＞1,3,6-trihydroxy-2-methylanthraquinone ＞ purpurin. The overall quality of Rubiae Radix in Shaanxi, Henan, and Anhui Province was good. Conclusion The method is convenient, accurate, and easy to promote, which can comprehensively and systematically evaluate the quality of Rubiae Radix.

R286.02

河南省医学科技攻关计划省部共建指导性计划重点项目（SBGJ202002105）