目的 建立HPLC法同时测定糖痹通络颗粒中原儿茶酸、羟基红花黄色素A、对香豆酸、阿魏酸、β-蜕皮激素、络石苷。方法 采用ACE 5 C₁₈-AR色谱柱（250 mm×4.6 mm，5 μm）；流动相乙腈–0.1%磷酸水溶液，梯度洗脱；检测波长260 nm（原儿茶酸、β-蜕皮激素）、280 nm（对香豆酸、阿魏酸、络石苷）、360 nm（羟基红花黄色素A）；体积流量1 mL/min；柱温40 ℃；进样量5 μL。结果 原儿茶酸、羟基红花黄色素A、对香豆酸、阿魏酸、β-蜕皮激素、络石苷在各自线性范围内线性关系良好（r≥0.999 5），平均加样回收率分别为103.63%、96.93%、103.18%、97.09%、102.38%、100.08%，RSD值分别为1.01%、0.88%、1.27%、0.90%、1.35%、1.05%。结论 该方法简单准确、稳定可靠、重复性好，可用于糖痹通络颗粒的质量控制。

Objective To establish an HPLC method for the simultaneous determination of protocatechuic acid, hydroxysafflor yellow A, p-coumaric acid, erulic acid, β-ecdysterone, and tracheloside in Tangbi Tongluo Granules. Methods The analysis was performed on ACE 5 C₁₈-AR column (250 mm×4.6 mm, 5 μm). The mobile phase was acetonitrile - 0.1% phosphoric acid solution with gradient elution. The detection wavelength was set at 260 nm (protocatechuic acid and β-ecdysterone), 280 nm (p-coumaric acid, ferulic acid, and tracheloside), 360 nm (hydroxysafflor yellow A). The flow rate was 1.0 mL/min, temperature of column was set at 40 ℃, and the volume of injection was 5 μL. Results Protocatechuic acid, hydroxysafflor yellow A, p-coumaric acid, erulic acid, β-ecdysterone, and tracheloside showed good linear relationships within their respective linear ranges (r ≥ 0.999 5), whose average recoveries were 103.63%, 96.93%, 103.18%, 97.09%, 102.38%, and 100.08% with RSD values of 1.01%, 0.88%, 1.27%, 0.90%, 1.35%, and 1.05%, respectively. Conclusion The method is simple accurate, stable reliable, and repeatable, which can be used to control the quality of Tangbi Tongluo Granules.

R286.02

国家自然科学基金资助项目（82174293，81774117）；国家自然科学基金青年科学基金资助项目（82004286）；江苏省中医药科技发展计划项目（ZD202208）；江苏省中医药科技发展计划项目（ZT202206）