目的 探讨芩百清肺浓缩丸对肺炎支原体CARDs TX毒素蛋白表达的抑制作用。方法 将Balb/c小鼠随机分为对照组、模型组、阿奇霉素组及芩百清肺浓缩丸4.6、2.3、1.15 g/kg组，每组9只。除对照组外，其余各组小鼠进行支原体滴鼻，每只滴入20 μL 1×10⁶ CCU肺炎支原体菌液，建立肺炎支原体感染模型。支原体肺炎模型建立成功后各组分别ig小鼠相应药物，连续ig 7 d，末次给药结束后进行引咳实验，并处死小鼠，取血液与肺组织。显微镜下观察各组小鼠肺组织苏木精–伊红（HE）染色、Masson染色、免疫组化后的肺组织变化；RT-PCR法检测各组小鼠肺组织MPN372、BDKRB2表达量。结果 与模型组相比，芩百清肺浓缩丸可显著降低肺炎支原体小鼠咳嗽次数；芩百清肺浓缩丸组小鼠肺组织结构明显得到改善，结构清晰，组织形态规整；芩百清肺浓缩丸组小鼠肺组织纤维化得到缓解；芩百清肺浓缩丸组小鼠肺组织MPN372、BDKRB2 mRNA表达显著降低（P＜0.05）。结论 芩百清肺浓缩丸通过降低肺组织中CARDs TX、BDKRB2的表达，减缓咳嗽症状，治疗肺炎支原体肺炎。

Objective To investigate the inhibitory effect of Qinbai Qingfei Concentrated Pills on the expression of CARDs TX toxin protein of mycoplasma pneumoniae. Methods Balb/c mice were randomly divided into control group, model group, azithromycin group, and Qinbai Qingfei Concentrated Pills (4.6, 2.3 and 1.15 g/kg) groups, with 9 mice in each group. Except the control group, mice in other groups received mycoplasma nasal drops, each of which was infused with 20 μL 1×10⁶ CCU mycoplasma pneumoniae solution, and the mycoplasma pneumoniae infection model was established. After the successful establishment of mycoplasma pneumonia model, mice in each group were ig treated with the corresponding drug for 7 d. After the last administration, the cough inducing experiment was carried out, and the mice were killed, and blood and lung tissues were collected. The changes of lung tissue after HE staining, Masson staining, and immunohistochemistry were observed under microscope. The expression levels of MPN372 and BDKRB2 in lung tissues of each group were detected by RT-PCR. Results Compared with the model group, Qinbai Qingfei Concentrated Pills group can significantly reduce the cough times of mycoplasma pneumoniae mice. In Qinbai Qingfei Concentrated Pills group, the lung tissue structure was obviously improved, the structure was clear and the tissue morphology was regular. The pulmonary fibrosis of mice in Qinbai Qingfei Concentrated Pills group was alleviated. The mRNA expressions of MPN372 and BDKRB2 in lung tissue of Qinbai Qingfei Concentrated Pills group were significantly decreased (P < 0.05). Conclusion Qinbai Qingfei Concentrated Pills can reduce the expression of CARDs TX and BDKRB2 in lung tissue to relieve cough symptoms and treat mycoplasma pneumoniae pneumonia.

R974；R285

国家自然科学基金项目（82174060）；黑龙江省自然科学基金项目（LH2021H074）；黑龙江省卫生健康委科研课题（20210202040011）