目的 研究六神丸对小鼠口腔溃疡的疗效及可能的作用机制。方法 将32只C57BL/6JGpt级小鼠随机分成对照组、模型组、六神丸组、桂林西瓜霜组，每组8只。于小鼠口腔左侧颊部黏膜上使用氢氧化钠片状晶体灼烧构建口腔溃疡模型。对口腔溃疡造模成功的小鼠分别外敷涂抹给予六神丸和桂林西瓜霜粉末0.375 g，连续5 d。观察小鼠口腔溃疡的恢复状态，用ELISA试剂盒检测血清白细胞介素（IL）-6、IL-8水平。取口腔溃疡部位组织，将溃疡组织制备匀浆，用二喹啉酸（BCA）法测定总蛋白浓度，并用Western blotting方法检测细胞外调节蛋白激酶（ERK）磷酸化水平。结果 与模型组比较，六神丸组小鼠口腔溃疡部位组织恢复情况较好，IL-6、IL-8水平显著降低（P＜0.05），局部组织中ERK磷酸化水平降低。结论 六神丸对氢氧化钠灼烧的口腔溃疡小鼠模型具有一定治疗作用，且可能的机制是通过降低血液中IL-6和IL-8水平，进而导致局部组织中ERK磷酸化水平降低改善病理状态。

Objective To study the treatment effect of Liushen Pills for oral ulcer in mice. Methods Thirty-two C57BL/6JGpt mice were randomly divided into control group, model group, Liushen Pills group, and Guilin Watermelon Cream group, each grop had 8 mice. The oral ulcer model was constructed by burning the left buccal mucosa of the oral cavity of mice with sodium hydroxide flake crystal. Mice with successfully constructed oral ulcer were treated with Liushen Pills and Guilin Watermelon Cream for 5 d. To observe the recovery state of oral ulcer in mice, serum levels of IL-6 and IL-8 were detected by ELISA kit. The ulcerated tissues of oral cavity were taken and homogenized. The total protein concentration was determined by BCA method, and the phosphorylation level of extracellular regulated protein kinase (ERK) was detected by Western blotting method. Results Compared with model group, the oral ulcer tissues of mice in Liushen Pills group and Guilin Watermelon Cream group recovered better, the levels of IL-6 and IL-8 were significantly decreased (P < 0.05), and the phosphorylation level of ERK in local tissues was decreased. Conclusion Liushen Pills have a certain therapeutic effect on the NaOH burning oral ulcer mouse model, and the action was performed by decreasing the IL-6 and IL-8 in blood circulation and thus down-regulate the ERK1/2 phsphorlation level in local tissue.

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