目的 建立华山参的UPLC指纹图谱，鉴别其正品和混伪品。方法 建立华山参的UPLC指纹图谱，对相似度进行分析，采用聚类分析、主成分分析、偏最小二乘-判别分析法对共有峰进行分析。结果 建立的指纹图谱共标定出15个共有峰。华山参正品的相似度均在0.867以上，混伪品的相似度均低于0.555。30批华山参样品聚为3类，正品与伪品、主产地与非主产地华山参药材被显著区分，并筛选出4个差异性成分。结论 建立的UPLC指纹图谱能有效鉴别华山参及其混伪品，为华山参药材的质量评价和真伪鉴别提供参考。

Objective To establish an UPLC fingerprint of Physochlainae Radix to identify Physochlainae Radix and its adulterants. Methods UPLC fingerprint of Physochlainae Radix was established, the similarity was analyzed. The common peaks were analyzed by cluster analysis, principal component analysis, and partial least squares-discriminant analysis. Results A total of 15 characteristic peaks were calibrated in the established fingerprint. Similarities of Physochlainae Radix were all above 0.867, and the similarity of adulterants were all less than 0.555. Thirty batches of Physochlainae Radix samples were clustered into 3 categories. Superior and inferior products of Physochlainae Radix, as well as primary and non-primary producing areas, were significantly distinguished, and 4 quality difference markers were screened. Conclusion The established UPLC fingerprint can effectively identify Physochlainae Radix and its adulterants, providing reference for quality evaluation and identification of Physochlainae Radix medicinal materials.

R286.02

现代中医药海河实验室科技项目（22HHZYJC00005）