目的 探讨五味子提取物对小鼠银屑病样皮损的抑制作用及机制。方法 使用咪喹莫特乳剂建立小鼠银屑病背部皮损模型，将小鼠分为对照组、模型组、他克莫司5 mg/kg组以及五味子提取物0.05、0.25 mg/kg组，并于造模后在小鼠背部皮肤涂抹相应药物。取小鼠背部皮肤组织，分析皮损症状，对其进行苏木精–伊红（HE）染色，并观察测定表皮厚度。免疫组织化学测定淋巴细胞表面分子（CD-8）、环氧化酶-2（COX-2）、细胞外调节蛋白激酶（ERK）、磷酸化细胞外调节蛋白激酶（p-ERK）的表达情况。建立3D皮肤模型，使用双氧水造模后，噻唑蓝（MTT）检测五味子提取物抗氧化修复活性、酶联免疫吸附法检测皮肤模型组织中超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的酶活力和炎症因子白细胞介素-6（IL-6）的含量。结果 五味子提取物可以缓解由咪喹莫特乳剂诱导的小鼠银屑病皮肤损伤、红斑、炎症，改善其皮损处组织细胞情况，降低银屑病模型小鼠表皮厚度（P＜0.05）；五味子提取物可以下调银屑病模型小鼠皮肤组织中COX-2、ERK、p-ERK、CD-8的表达（P＜0.05、0.01）；3D皮肤模型检测结果显示，五味子提取物可以减缓双氧水对3D皮肤细胞的氧化损伤作用，降低皮肤组织细胞中IL-6的表达，同时可以提高SOD和CAT酶的活性（P＜0.05、0.01）。结论 五味子提取物对小鼠银屑病样皮损具有一定抑制作用，可能与通过免疫调节来抑制CD-8、COX-2、ERK、p-ERK的表达和缓解氧化应激损伤有关。

Objective To investigate the inhibitory effect and mechanism of Schisandra Chinensis Fructus extract on psoriasis-like skin lesions in mice. Methods Mice were divided into control group, model group, tacrolimus 5 mg/kg group, and Schisandra Chinensis Fructus extract 0.05 and 0.25 mg/kg groups. After modeling, the mice were smeared with the corresponding drug on the skin of the back of the mice. The skin tissue of the back of mice was collected, the symptoms of skin lesions were analyzed, and the skin thickness was observed and measured by hematoxylin-eosin (HE) staining. The expressions of CD-8, COX-2, ERK, and p-ERK were determined by immunohistochemistry. The 3D skin model was established, and the antioxidant repair activity of Schisandra Chinensis Fructus extract was detected by thiazole blue (MTT), and the enzyme activity of SOD, CAT, and the content of inflammatory factor IL-6 in the skin model tissues were detected by enzymelinked immunosorbent assay (ELISA). Results Schisandra Chinensis Fructus extract can relieve the skin injury, erythema and inflammation induced by imiquimod emulsion, improve the tissue and cell condition of the lesions, and reduce the epidermal thickness of psoriasis model mice (P < 0.05). The extract of Schisandra Chinensis Fructus extract could down-regulate the expressions of COX-2, ERK, p-ERK, and CD-8 in the skin tissue of psoriasis model mice (P < 0.05, 0.01). 3D skin model test results showed that Schisandra Chinensis Fructus extract can slow down the oxidative damage of hydrogen peroxide on 3D skin cells, reduce the expression of IL-6 in skin tissue cells, and increase the activities of SOD and CAT enzymes (P < 0.05, 0.01). Conclusion The extract of Schisandra Chinensis Fructus extract showed certain inhibitory effect on psoriasis lesions in mice, which may be related to the inhibition of CD-8, COX-2, ERK, and p-ERK expression and the relief of oxidative stress injury through immune regulation.

R285.5

国家自然科学基金资助项目（22178070）