目的 探讨阿司匹林对人鼻咽癌CNE-1、5-8F细胞增殖、迁移与侵袭的影响和机制。方法 将人鼻咽癌CNE-1、5-8F细胞株分为对照组和阿司匹林（0.00、1.25、2.50、5.00、10.00mmol/L）处理组。噻唑蓝（MTT）法检测不同浓度阿司匹林作用24、48、72h后对CNE-1、5-8F细胞的增殖能力，以半数抑制浓度（IC₅₀）作为后续实验药物处理浓度。集落形成实验检测CNE-1和5-8F细胞增殖能力，Transwell实验检测CNE-1和5-8F细胞迁移及侵袭能力的变化，Western blotting法检测CNE-1和5-8F细胞中蛋白激酶B（Akt）、磷脂酰肌醇3-激酶（PI3K）、波形蛋白（Vimentin）、E-钙黏蛋白（E-cadherin）、Snail蛋白相对表达水平。结果 人鼻咽癌CNE-1、5-8F细胞分别经1.25、2.50、5.00、10.00mmol/L阿司匹林处理后，细胞增殖均受到不同程度抑制，且呈时间及浓度相关性，IC₅₀分别为3.3、2.7mmol/L。与对照组相比，经阿司匹林处理后，鼻咽癌CNE-1、5-8F细胞的细胞增殖和集落形成能力明显下降（P＜0.01）。与对照组相比，经阿司匹林处理后，划痕愈合率显著降低（P＜0.05）。Trasnwell迁移实验结果表明，与对照组相比，阿司匹林处理组鼻咽癌细胞CNE-1和5-8F 48h穿过小室的数目显著减少（P＜0.05、0.001）。与对照组相比，阿司匹林处理组CNE-1、5-8F细胞的E-cadherin蛋白表达显著增加（P＜0.05），PI3K、Akt、Vimentin、Snail蛋白表达显著下降（P＜0.05、0.01）。结论 阿司匹林可通过上调E-cadherin蛋白的表达、抑制上皮间质转化进程从而抑制人鼻咽癌CNE-1、5-8F细胞迁移侵袭，同时下调PI3K、Akt、Vimentin、Snail蛋白的表达进而抑制CNE-1、5-8F细胞增殖、迁移和侵袭能力。

Objective To investigate the effect and mechanism of aspirin on the proliferation, migration and invasion of CNE-1 and 5-8F cells in human nasopharyngeal carcinoma. Methods Human nasopharyngeal carcinoma cell lines CNE-1 and 5-8F were divided into control group and aspirin (0.00, 1.25, 2.50, 5.00, and 10.00 mmol/L) treatment group. The proliferation capacity of CNE-1 and 5-8F cells was detected by MTT assay after the treatment of aspirin with different concentrations for 24, 48, and 72 h, IC₅₀ was determined as the concentration for subsequent experimental drug treatment. The proliferation ability of CNE-1 and 5-8F cells was detected by colony formation assay, and the migration and invasion ability of CNE-1 and 5-8F cells were detected by Transwell assay. The relative expression levels of Akt, PI3K, Vimentin, E-cadherin, and Snail in CNE-1 and 5-8F cells were detected by Western blotting. Results Human nasopharyngeal carcinoma CNE-1 and 5-8F cells were treated with 1.25, 2.50, 5.00, and 10.00 mmol/L aspirin, respectively, and the cell proliferation was inhibited in different degrees, which showed time and concentration correlation, and IC₅₀ were 3.3 and 2.7 mmol/L, respectively. After treatment with aspirin, the cell proliferation and colony formation ability of nasopharyngeal carcinoma CNE-1 and 5-8F cells were significantly decreased compared with the control group (P<0.01). Compared with the control group, the scratch healing rate in the aspirin treatment group was significantly decreased (P<0.05). Trasnwell migration assay showed that compared with the control group, the number of nasopharyngeal carcinoma cells CNE-1 and 5-8F passing through the compartment at 48 h in aspirin treatment group was significantly reduced (P<0.05, 0.001). Compared with the control group, the expression of E-cadherin in CNE-1 and 5-8F cells in aspirin treatment group was significantly increased (P<0.05), and the expression of PI3K, Akt, Vimentin and Snail was significantly decreased (P<0.05, 0.01). Conclusions Aspirin can inhibit the migration and invasion of human nasopharyngeal carcinoma CNE-1 and 5-8F cells by up-regulating the expression of E-cadherin protein and inhibiting the process of epithelial mesenchymal transformation, while down-regulating the expressions of PI3K, Akt, Vimentin and Snail proteins, thus inhibiting the proliferation, migration and invasion of CNE-1 and 5-8F cells.

R965

国家自然科学基金资助项目（81960172）；广西自然科学基金资助项目（2020GXNSFAA238014）；桂林医学院博士科研启动项目（20501019035）