目的 建立气相色谱–氢火焰离子化检测器（GC-FID）法同时测定消癥丸中莪术酮、藁本内酯和α-香附酮的方法。方法 采用HP-5MS毛细管柱（30 m×0.25 mm×0.25 μm），FID检测器：280℃，进样口温度240 ℃；程序恒温130 ℃，保持45 min；载气为N₂，柱体积流量1.5 mL/min，不分流模式；进样量：1 μL。结果 莪术酮、藁本内酯、α-香附酮分别在1.684～168.400、2.496～249.600、2.180～218.000 μg/mL线性关系良好；平均回收率分别为97.98%、99.76%、96.06%，RSD值分别为1.62%、0.89%、1.29%。结论 该方法操作简单、重复性好，可为评价消癥丸的质量控制提供依据。

Objective To establish a method for simultaneous determining of curzerenone,ligustilide,and α-cyperone in Xiaozheng Pills by GC-FID method.Methods The separation was carried out on HP-5MS column (30 m×0.25 mm×0.25 μm).Detector was FID detector,and the detector temperature was 280℃,and the inlet temperature was 240℃.The program temperature was 130℃ for 45 min.The carrier gas was nitrogen,and the flow rate was 1.5 mL/min with splitless inlet.The injection volume was 1 μL.Results The linear ranges of curzerenone,ligustilide,and α-cyperone were 1.684-168.400,2.496-249.600,2.180-218.000 μg/mL,respectively.The average recoveries of curzerenone,ligustilide,and α-cyperone were 97.98%,99.76%,and 96.06% with RSD values of 1.62%,0.89%,and 1.29%,respectively.Conclusion The established method is simple and reproducible,which can be used for the quality control in Xiaozheng Pills.

R286.02

江苏省科技成果转化专项资金项目(BA2012078)