目的 研究乳香及其炮制品对葡聚糖硫酸钠（DSS）诱导的小鼠炎症性肠病的保护作用。方法 采用DSS水溶液代替正常饮水建立炎症性肠病小鼠模型，将实验小鼠分为对照组、模型组、柳氮磺胺吡啶（300 mg/kg）组、生乳香（0.76 g/kg）组、醋乳香（0.76 g/kg）组、生乳香粪清液（0.2 mL）组、醋乳香粪清液（0.2 mL）组，分别进行给药治疗。观察和监测小鼠体质量、粪便性状、便血情况，进行疾病活动指数（DAI）评分；记录结肠长度；运用苏木精–伊红染色法（HE）观察炎症性肠病小鼠肠道组织的病理变化；酶联免疫吸附试验（ELISA）检测炎症性肠病小鼠血清中白细胞介素（IL）-4、IL-10、IL-6、IL-1β的水平。采用蛋白质免疫印迹法（Western blotting）测定炎症性肠病小鼠肠道组织中肠黏膜机械屏障蛋白的表达情况。结果 生乳香、醋乳香、生乳香粪清液组和醋乳香粪清液组均可改善DSS造成的小鼠体质量下降、粪便性状评分和便血情况，显著降低DAI评分；改善炎症性肠病小鼠肠道组织的病理状态；调节血清中的炎症因子水平；显著升高炎症性肠病小鼠肠道组织中咬合蛋白（occludin）、紧密连接蛋白1（claudin-1）、闭合小环蛋白1（ZO-1）蛋白表达水平。结论 乳香及其炮制品可改善炎症性肠病小鼠炎症反应，对肠黏膜屏障具有一定的保护作用，且粪菌移植效果更佳。

Objective To investigate the protective effects of Olibanum and its processed products on inflammatory bowel disease induced by dextran sulfate sodium (DSS) in mice.Methods DSS solution was used instead of normal drinking to establish inflammatory bowel disease mice model, experimental mice were divided into control group, model group, sulfasalazine (300 mg/kg) group, raw Olibanum (0.76 g/kg) group, vinegar Olibanum (0.76 g/kg) group, raw Olibanum purified feces (0.2 mL) group, and vinegar Olibanum purified feces (0.2 mL) group. The body weight, fecal traits, and hematochezia of mice were observed and monitored, and disease activity index (DAI) was scored. Colon length was recorded. HE staining was used to observe the pathological changes of intestinal tissues in mice with inflammatory bowel disease. The levels of interleukin-4, IL-10, IL-6, and IL-1β in serum of mice with inflammatory bowel disease were determined by ELISA. Western blotting was used to detect the protein expression in intestinal tissues of inflammatory bowel disease mice. Results Raw Olibanum, vinegar Olibanum, raw Olibanum purified feces, and vinegar Olibanum purified feces group could improve the decrease of body weight, fecal traits score, and blood in stool caused by DSS, and significantly reduce DAI score. To improve the pathological state of intestinal tissue in mice with inflammatory bowel disease. Regulating the level of inflammatory factors in serum. The expression levels of occludin, claudin-1, and ZO-1 in intestinal tissues of inflammatory bowel disease mice were significantly increased. Conclusion Olibanum and its processed products can improve the inflammatory response of inflammatory bowel disease mice and have certain protective effect on intestinal mucosal barrier.

R285.1

山西省卫生计生委科研课题计划项目(2019092);山西中医药大学科技创新能力培育计划(2019PY-154)