目的 建立HPLC-DAD法同时测定益气聪明丸中蔓荆子黄素、异阿魏酸、葛根素和芍药苷。方法Waters X-bridge C₁₈色谱柱（250 mm×4.6 mm，5 μm）；流动相：乙腈–0.5%磷酸溶液，梯度洗脱；检测波长：257 nm（蔓荆子黄素）、315 nm（异阿魏酸）、251 nm（葛根素）、230 nm（芍药苷）；体积流量：1.0 mL/min；柱温：25℃；进样体积：10 μL。结果 异阿魏酸、芍药苷、葛根素和蔓荆子黄素分别在0.098～9.830、0.199～19.900、0.239～23.900、0.237～23.700 μg/mL线性良好，平均回收率分别为99.57%、99.64%、99.13%、99.64%，RSD值分别为1.79%、1.35%、0.67%、1.43%。结论 建立的方法简便快捷、准确可靠，可用于益气聪明丸的质量控制。

Objective To establish an HPLC-DAD method for simultaneous determination of vitexicarpin, ferulic acid, puerarin, and paeoniflorin in Yiqi Congming Pills. Methods HPLC analysis was performed on Waters X-bridge C₁₈ column(250 mm×4.6 mm, 5 μm)with acetonitrile-0.5% phosphoric acid solution by gradient elution. The detection wavelength was set at 257 nm (vitexicarpin), 315 nm (ferulic acid), 251 nm (puerarin), and 230 nm (paeoniflorin). The flow rate was 1.0 mL/min, the column temperature was 25℃, and the sample volume was 10 μL. Results Vitexicarpin, ferulic acid, puerarin, and paeoniflorin showed good linear relationships in concentration range 0.098-9.830, 0.199-19.900, 0.239-23.900, and 0.237-23.700 μg/mL, respectively. The recoveries were 99.57%, 99.64%, 99.13%, and 99.64% with values of 1.79%, 1.35%, 0.67%, and 1.43%, respectively.Conclusion The method is simple, fast, accurate, and reliable, and can be applied to the quality control of Yiqi Congming Pills.

R286.02