目的 考察白头翁皂苷B₄对咪喹莫特诱导的银屑病模型小鼠的炎症及免疫相关指标的影响，探讨其对小鼠银屑病的治疗作用及其机制。方法 将56只BALB/c小鼠随机分成对照组，模型组，阿维A胶囊组（6 mg/kg），白头翁皂苷B₄低、中、高剂量（5、10、20 mg/kg）组，白头翁皂苷B₄乳膏组（20 mg/kg），每组各8只。通过局部涂抹5%咪喹莫特乳膏诱导小鼠银屑病模型，各组小鼠口服或涂抹相应药物，14 d后通过银屑病皮损面积及严重程度评分（PASI）评价小鼠皮损程度；HE染色观察皮肤组织病理学形态；血常规检测血液中免疫细胞的变化；酶联免疫吸附试剂（ELISA）检测小鼠皮肤中白细胞介素（IL）-6、IL-12、IL-17、IL-1β的水平。结果 与模型组相比，阿维A胶囊组，白头翁皂苷B₄ 5、10、20 mg/kg组，白头翁皂苷B₄乳膏组小鼠皮损组织棘层厚度减小，真皮层炎症细胞浸润减少，PASI评分、血液中的免疫细胞及皮肤组织中IL-6、IL-12、IL-17、IL-1β的表达水平均显著降低。结论 白头翁皂苷B₄对5%咪喹莫特诱导小鼠银屑病皮损具有明显的治疗作用，其机制可能是通过抑制IL-6、IL-12、IL-17、IL-1β等细胞因子表达发挥治疗作用。

Objective To investigate the effects of anemoside B₄ on inflammation and immune-related indexes in imiquimote-induced mouse psoriasis model mice, and to explore its therapeutic effect and mechanism. Methods A total of 56 BALB/c mice were randomly divided into control group, model group, Avirin Capsule group (6 mg/kg), anemoside B₄ low-dose, medium-dose and high-dose groups (5, 10, 20 mg/kg), Anemoside B₄ Cream group (20 mg/kg), with 8 mice in each group. The psoriasis model of mice was induced by topical application of 5% imiquimod cream, and the psoriasis degree of mice was evaluated by psoriasis area and severity score (PASI) 14 days later. The histopathological morphology of skin was observed by HE staining. Blood routine tests to detect changes in immune cells in the blood. The levels of IL-6, IL-12, IL-17, and IL-1β in mice skin were determined by enzyme-linked immunosorbent assay (ELISA). Results Compared with model group, the thickness of spinous layer of skin lesion tissue of mice in avitra capsule group, anemoside B₄ 5, 10, 20 mg/kg groups and Anemoside B₄ Cream group decreased, and inflammatory cell infiltration in dermis decreased. PASI score, the expression levels of IL-6, IL-12, IL-17, and IL-1β in blood immune cells and skin tissue were significantly decreased. Conclusion Anemoside B4 has obvious therapeutic effect on 5% imiquimod-induced psoriasis in mice, and its therapeutic mechanisms may be through by inhibiting the expression of cytokines such as IL-6, IL-12, IL-17, IL-1β and other cytokines.

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广西创新驱动发展项目（桂科AA19254024）；广西科技计划项目（桂科AB20025002）