目的 建立高效液相色谱-一测多评法（HPLC-QAMS）测定肝爽颗粒中虎杖苷、白藜芦醇、氧化芍药苷、芍药苷和柴胡皂苷a、b₁、d的方法。方法 采用Agilent SB-C₁₈色谱柱（250 mm×4.6 mm，5μm）；流动相为乙腈-0.1%磷酸溶液，梯度洗脱；检测波长分别为306 nm（0~17 min，测定虎杖苷和白藜芦醇）、230 nm（17~28 min，测定氧化芍药苷和芍药苷）、210 nm（28~55 min，测定柴胡皂苷a、b₁、d）；体积流量1.0 mL/min；柱温30℃；进样量10 μL。以芍药苷为内参物，建立其他6个成分的相对校正因子（RCF）计算。结果 虎杖苷、白藜芦醇、氧化芍药苷、芍药苷和柴胡皂苷a、b₁、d分别在6.37~159.25、4.91~122.75，1.88~47.00、7.99~199.75、0.56~14.00、2.19~54.75、2.87~71.75μg/mL线性关系良好，平均回收率分别为100.08%、98.95%、97.87%、99.52%、96.97%、98.88%、99.07%，RSD值分别为0.72%、1.1%、1.4%、0.99%、1.2%、1.2%、0.84%。肝爽颗粒中各成分的一测多评法所得结果与外标法结果无明显差异。结论 所建立的HPLC-QAMS法可用于肝爽颗粒中虎杖苷、白藜芦醇、氧化芍药苷、芍药苷和柴胡皂苷a、b₁、d的同时测定。

Objective To establish an high performance liquid chromatography-quantitative analysis of multi-component by single marker (HPLC-QAMS) method for determination of polydatin, resveratrol, oxypaeoniflorin, paeoniflorin, saikosaponin a, saikosaponin b₁, and saikosaponin d in Ganshuang Granules. Methods Agilent SB-C₁₈ (250 mm×4.6 mm,5 μm) was used as the column with acetonitrile-0.1% phosphoric acid as the mobile phase by gradient elution. The detection wavelength were 306 nm for polydatin and resveratrol, 230 nm for oxypaeoniflorin and paeoniflorin, and 210 nm for saikosaponin a, saikosaponin b₁, and saikosaponin d. The flow rate was 1.0 mL/min, the column temperature was 30℃, and the injection volume was 10 μL. With paeoniflorin as the internal reference, the relative correction factors (RCF) of the other six constituents were calculated, and the content were determined. Results Polydatin, resveratrol, oxypaeoniflorin, paeoniflorin, saikosaponin a, saikosaponin b₁, and saikosaponin d showed good linear relationships within the ranges of 6.37-159.25, 4.91-122.75, 1.88-47.00, 7.99-199.75, 0.56-14.00, 2.19-54.75, 2.87-71.75 μg/mL, whose average recoveries (RSDs) were 100.08% (0.72%), 98.95% (1.1%), 97.87% (1.4%), 99.52% (0.99%), 96.97% (1.2%), 98.88% (1.2%), and 99.07% (0.84%), respectively. There was no significant difference on components contents between the quantitative results of HPLC-QAMS and those of external standard method (ESM). Conclusion HPLC-QAMS method can be used for the simultaneous determination of polydatin, resveratrol, oxypaeoniflorin, paeoniflorin, saikosaponin a, saikosaponin b₁, and saikosaponin d in Ganshuang Granules.

R286.02