目的 通过网络药理学方法探究黄芪治疗肺结核的作用机制。方法 运用TCMSP数据库筛选黄芪的活性成分及其作用靶点；借助GeneCard数据库筛选肺结核疾病靶点，并获得交集靶点；利用STRING数据库构建靶点蛋白相互作用网络；将交集靶点进行基因本体（gene ontology，GO）和京都基因与基因组百科全书（kyoto encyclopedia of genes and genomes，KEGG）富集分析。结果 筛选得到黄芪的活性成分20个，活性成分主要包括槲皮素、异鼠李素、异黄酮等，其治疗肺结核的相关靶点为TNF、RELA、IL-6、AKT1、MAPK1、MAPK14、IL-1B、MAPK8、IL-10、IFNG、STAT1等；KEGG分析结果表明黄芪可能通过干预Toll样受体信号通路、结核病通路、T细胞受体信号通路及TNF信号通路等发挥治疗肺结核的作用。结论 黄芪治疗肺结核具有多成分–多靶点–多通路的特点，多与炎症、免疫机制相关，为黄芪的实验研究和临床合理用药提供科学依据。

Obtective To investigate the pharmacology mechanism of Astragali Radix for pulmonary tuberculosis based on network pharmacology.Methods The TCMSP database was used to screen the active ingredients of Astragali Radix and their targets, the GeneCard database was used to screen the pulmonary tuberculosis targets and obtain the intersecting targets, the STRING database was used to construct the target protein interaction network, the intersecting targets were subjected to GO and KEGG enrichment analysis.Results Twenty active components of Astragali Radix were screened, the active ingredients mainly include quercetin, isorhamnetin, and isoflavone, and their relevant targets for the treatment of pulmonary tuberculosis were TNF, RELA, IL-6, AKT1, MAPK1, MAPK14, IL-1B, MAPK8, IL-10, IFNG, STAT1, etc. The results of KEGG analysis indicated that Astragali Radix may interfere with the Toll-like receptor signaling pathway, tuberculosis pathway, T-cell receptor signaling pathway and TNF signaling pathway to play a role in treatment of pulmonary tuberculosis.Conclusion Astragali Radix has multi-component-multi-target-multi-pathway characteristics in the treatment of pulmonary tuberculosis, mostly related to inflammation and immune mechanism, which provides scientific basis for experimental research and rational clinical use of astragalus.

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昆明市卫生科技人才培养项目（2020-SW[后备]-60）；昆明市卫生健康委员会卫生科研课题项目（2021-16-01-0010）