目的 利用网络药理学的方法探究丹香冠心注射液治疗心绞痛、心肌梗死的作用机制及配伍规律。方法 选取丹香冠心注射液中丹参中有机酸类成分丹参素、原儿茶醛，丹酚酸类成分丹酚酸B、丹酚酸D、迷迭香酸及降香中挥发油类成分橙花叔醇、氧化橙花叔醇、香兰素为研究对象，利用Swiss Target Prediction、TCMSP数据库对8个化合物的潜在作用靶点进行预测。借助GeneCards、OMIM、DisGeNET等数据库收集与心绞痛、心肌梗死相关的靶点，将化合物靶点和疾病靶点取交集，然后通过String、Omicsbean等数据库筛选核心靶点及通路，借助Cytoscape软件构建丹参、降香“药材–成分–靶点–通路–药理作用”网络图，并利用分子对接技术对预测结果进行验证。最后通过数据整合分析，剖析丹香冠心注射液的作用机制及配伍规律。结果 8个化合物通过作用于35个核心靶点干预了33条相关信号通路，发挥抗炎、外周镇痛、中枢镇痛、抗血栓、扩张血管、保护血管内皮、神经保护、抗氧化应激等作用。各类成分既有共同的作用靶点群及通路群，又各有偏重，各通路群间通过共有靶点连接，显示出不同成分间的多靶点、多途径的协同作用。结论 丹参可通过抗炎、外周镇痛、抗血栓、保护血管内皮功能、扩张血管等发挥抗炎、活血、祛瘀等功效，起到君药的作用。降香除调节上述相关过程外还可通过作用于中枢镇痛相关受体从而发挥理气止痛、化瘀止血的功效，辅助君药丹参起到臣药作用。

Objective To explore the mechanism and compatibility rules of Danxiang Guanxin Injection for the treatment of angina pectoris and myocardial infarction by network pharmacology. Methods Salvianic acid A and protocatechuic aldehyde of organic acids, and salvianolic acid B, salvianolic acid D, rosmarinic acid of salvianolic acid compounds in Salvia miltiorrhiza, and nerolidol, nerolidol oxide, and vanillin of volatile oil in Dalbergiae Odoriferae Lignum were studied. The potential targets of 8 compounds were predicted by Swiss Target Prediction and TCMSP database. The disease targets related to angina pectoris and myocardial infarction were collected by searching GeneCards, OMIM, DisGeNET databases, and the compound targets and disease targets were intersected. Then the core targets and pathways were screened by String and Omicsbean. The network of "herb-compound-target-pathway- pharmacological action" of Salvia miltiorrhiza and Dalbergiae Odoriferae Lignum was constructed by Cytoscape, and the results were verified by molecular docking. Finally, mechanism and compatibility of Danxiang Guanxin Injection were analyzed by data integration.Results The 8 compounds can act on 35 related targets and 33 related pathways, mainly related to anti-inflammation, peripheral analgesia, central analgesia, antithrombotic, vasodilation, protection of vascular endothelium, neuroprotection, antioxidant stress and so on. All kinds of compounds not only have common target groups and pathway groups, but also have their own emphasis. Through the connection of common targets, each pathway group shows the synergistic effect of multi-targets and multi-pathways among different components. Conclusion Salvia miltiorrhiza can exert anti-inflammation, blood activating and blood stasis removing effects through anti-inflammatory, peripheral analgesia, antithrombotic, protecting vascular endothelium and dilating blood vessels. In addition to regulating the above-mentioned related processes, Dalbergiae Odoriferae Lignum can also exert the effect of regulating qi and relieving pain, removing blood stasis and hemostasis by acting on the related receptors of central analgesia, assist the monarch drug Salvia miltiorrhiza to play the role of subject medicine.

R286.2

天津市科技计划项目（20KPHDRC00020）