目的 优选芪苓益肾颗粒的提取工艺。方法 以黄芪甲苷得率、总多糖得率、干膏得率的综合评分为评价指标，采用层次分析法（AHP）确定这3个指标权重系数，设计L₉（3⁴）正交试验筛选最佳加水量、提取次数和提取时间，并进行验证试验。结果 以AHP法确定的权重系数合理有效。筛选的最优提取工艺为加8倍量水，提取3次，每次1 h。3次验证试验结果显示，黄芪甲苷、总多糖、干膏得率的平均值分别为0.10%、2.51%、43.09%；平均综合评分为99.88，RSD值为0.03%。结论 正交试验设计结合AHP确定的多指标权重系数法可较好地优化芪苓益肾颗粒的提取工艺。

Objective To optimize the extraction technology of Qiling Yishen Granules. Methods The comprehensive scores of astragaloside IV yield, total polysaccharides yield, and dry extract yield were used as the evaluation index, and the weight coefficient of these indexes was determined by analytic hierarchy process (AHP). L₉ (3⁴) orthogonal test was designed to screen water volume, extraction times, and extraction time, and validation tests were carried out. Results The weight coefficient determined by AHP method was reasonable and effective. The optimized extraction technology was to add 8-fold water, extracting 3 times, and 1 h each time. The results of three validation tests showed that the average yields of astragaloside IV, total polysaccharides, and dry paste were 0.10%, 2.51%, and 43.09%, respectively. Average comprehensive score was 99.88 with RSD value 0.03%. Conclusion Orthogonal test and multi-index weighting analysis method could be used to optimized extraction process of Qiling Yishen Granules.

R284.2

河南省医学科技攻关计划项目（LHGJ20191429）