目的 对甘草泻心汤中化学成分进行鉴定。方法 对甘草泻心汤、单味药汤剂和混合对照品进行HPLC分析。采用Agela venusil MP C₁₈（2）色谱柱（250 mm×4.6 mm，5 μm）；流动相：0.2%甲酸水溶液–乙腈，梯度洗脱，检测波长265、327 nm，体积流量1.0 mL/min，柱温25℃，进样量10 μL。液路采用三通分流0.2 mL/min进入ESI源，在正、负离子模式下进行质谱检测。采用甲酸钠溶液作为内参校准液每针校正。根据对照品、文献报道和数据库数据推测鉴定相关色谱峰的化合物和单味药来源。结果 提取出85个基峰离子，根据元素分析推测出其化学式，其中80个推测出了可能的化合物结构信息，采用对照品确证了6个成分，其中归属于甘草、黄芩、大枣、黄连、干姜、半夏分别为40、31、13、11、5、1个成分。结论 HPLC-QTOF/MS作为一种高选择性、高分离度、高灵敏度的分析手段能有效地鉴定甘草泻心汤中化学成分。

Objective To identify the chemical constituents in Gancao Xiexin Decoction. Methods GancaoXiexin Decoction, single herb decoction and mixed reference substance were analyzed on HPLC C₁₈ column (250 mm×4.6 mm, 5 μm) with the mobile phase 0.2% formic acid- acetonitrile at a flow rate of 1.0 mL/min. Detection wavelengths were set at 265 and 327 nm, volume flow rate was 1.0 mL/min, column temperature was 25 ℃, and sample injection volume was 10 μL. The fluid was infused into the ESI source at a flow rate of 0.2 mL/min by a tees，and was scanned in positive and negative ion mode by MS2. Sodium formate solution was used as internal standard. The compound attribution of related chromatographic peaks and the source of single herb were deduced and identified by comparison with the standard, literature, and database. Results Base peak chromatogram ions (85) were extracted and chemical formulas were deduced by elemental analysis, of which 80 constituents were deduced, and 6 constituents were confirmed using reference substances. Glycyrrhizae Radix et Rhizoma, Scutellariae Radix, Jujubae Fructus, Coptidis Rhizoma, Zingiberis Rhizome, and Pinelliae Rhizoma contributed 40, 31, 13, 11, 5, and 1 constituents, respectively. Conclusion HPLC-QTOF/MS can be applied in identification of constituents in Gancao Xiexin Decoction as a high selectivity, high resolution, and high sensitivity method.

R286.02

全国中药特色技术传承人才培训项目（国中医药人教函[2018]204号）；第六批全国老中医药专家学术经验继承工作；天津市卫健委中医、中西医结合科研基金项目（2019039）；天津市卫健委中医、中西医结合科研基金项目（2019050）；天津中医药大学第二附属医院横向科研项目（20191201）