目的 运用网络药理学方法探讨祛痹痛风饮治疗痛风的活性成分、作用靶点及其潜在的治疗作用机制。方法 基于中药系统药理学数据库与分析平台（TCMSP）获取祛痹痛风饮的活性成分和作用靶点，结合Uniprot数据库对靶点进行标准化处理。采用Cytoscape 3.7.2软件绘制出祛痹痛风饮的活性成分-痛风靶点网络。通过GeneCards、OMIM、TTD、DRUGBANK数据库获得痛风的疾病靶点。将药物的作用靶点与疾病靶点进行交集，获得共同靶点。将交集靶点应用STRING数据库构建蛋白-蛋白相互作用网络图（PPI），通过DAVID数据库对共同靶点进行基因本体（GO）功能富集分析和京都基因与基因组百科全书（KEGG）通路富集分析，并将结果进行可视化处理。结果 共预测到祛痹痛风饮中191个活性成分，87个与痛风共同的靶点。GO功能富集分析显示与炎症反应、细胞对脂多糖的应答、基因表达的正调控等相关。KEGG通路富集分析结果显示主要涉及Toll样受体信号通路、NOD样受体信号通路、NF-κB信号通路等方面。结论 祛痹痛风饮可能通过促进尿酸排泄、抑制炎症因子的释放减轻、炎症反应发挥治疗痛风的作用，为深入研究祛痹痛风饮治疗痛风的作用机制提供了理论参考。

Objective To study the active ingredient, target and possible mechanism of Qubi Tongfeng Yin in treatment of gout by network pharmacological method. Methods TCMSP database was used to obtain the active ingredient and target of Qubi Tongfeng Yin, and the target was standardized by Uniprot database. Cytoscape3.7.2 software was used to draw the network of drug active ingredient and target of gout. The disease targets of gout were obtained through Genecards, OMIM, TTD, and DRUGBANK databases. The drug target is intersected with the disease target to obtain the drug and disease common target. PPI was constructed for the intersection targets using String database. Gene ontology (GO) biological process analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed for the common targets using David database, and the results were visualized. Results A total of 191 active components of Qubi Tongfeng Yin were predicted in this study, and 87 common targets of Qubi Tongfeng Yin were predicted. The results of GO biological process showed that it was related to inflammatory response, cellular response to lipopolysaccharide, and positive regulation of gene expression. KEGG pathway enrichment analysis showed that it mainly involved Toll-like receptor signaling pathway, NOD-like receptor signaling pathway, NF-κB signaling pathway and so on. Conclusion Qubi Tongfeng Yin may play a role in treatment of gout by promoting uric acid excretion and inhibiting the release of inflammatory factors to reduce inflammatory response, which provides a theoretical basis for the further study of the mechanism of Qubi Tongfeng Yin in treatment of gout.

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国家中医药管理局全国名中医崔公让传承工作室建设项目（国中医药办人教函[2018]119号）；河南省首批青苗人才培养项目（豫中医科教[2018]16号）；河南省中医药科学研究专项课题（2019JDZX2103）