目的 建立HPLC法同时测定炎可宁胶囊中盐酸小檗碱、黄芩苷、大黄酸、大黄素、大黄酚、大黄素甲醚。方法 以Supelco Discovery C₁₈（250 mm×4.6 mm，5 μm）为色谱柱，甲醇–0.1%磷酸水溶液为流动相，梯度洗脱，检测波长为280 nm，柱温30℃，体积流量为0.8 mL/min，进样量10 μL。结果 6种成分在各自线性范围内关系良好（r²≥0.999），平均加样回收率99.0%～105.1%，RSD 1.41%～2.36%。结论 该方法简便准确，重复性好，可用于炎可宁胶囊的质量控制。

Objective To establish an HPLC method for the simultaneous determination of berberine hydrochloride, baicalin, rhein, emodin, chrysophanol, and emodin methyl ether in Yankening Capsules. Methods The Supelco Discovery C₁₈ (250 mm×4.6 mm, 5 μm) was used as the chromatographic column with mobile phase of methanol-0.1% phosphoric acid aqueous solution in gradient elution. The detection wavelength was set at 280 nm. The flow rate was 0.8 mL/min and the column temperature was 30℃. Results The six components had a good relationship within their respective linear ranges (r² ≥ 0.999). The average recovery rate of sample addition was 99.0%-105.1%, and the RSD was 1.41%-2.36%. Conclusion The method is simple, accurate, reproducible, and can be used for the quality control of Yankening Capsules.

R284.1