目的 建立高效液相色谱-电喷雾检测器（HPLC-CAD）法同时测定川芎茶调散中绿原酸、阿魏酸、阿魏酸松柏酯、洋川芎内酯A、Z-藁本内酯、欧前胡素、异欧前胡素。方法 采用资生堂Capcell Pak MGⅡC₁₈色谱柱（250 mm×4.6 mm，5 μm）；流动相：80%甲醇-10 mmol/L甲酸铵，梯度洗脱；体积流量：1.0 mL/min；柱温：40 ℃；CAD雾化器温度：35 ℃，采集频率：10 Hz，过滤常数：5.0；进样体积：20 μL；稀释剂：70%甲醇。结果 绿原酸、阿魏酸、阿魏酸松柏酯、洋川芎内酯A、Z-藁本内酯、欧前胡素、异欧前胡素的质量浓度分别在0.51～10.20 μg/mL（r=0.999 4），0.53～10.60μg/mL（r=0.999 3），2.52～50.40μg/mL（r=0.999 5），5.05～101.00μg/mL（r=0.999 4），5.01～100.20μg/mL（r=0.999 8），1.02～20.40μg/mL（r=0.9995）、0.62～12.40 μg/mL（r=0.9993）线性关系良好；平均回收率分别为99.46%、98.65%、98.48%、98.74%、98.60%、98.69%、98.58%，RSD值分别为1.62%、0.87%、1.03%、1.20%、1.25%、0.69%、0.66%。结论 方法准确、重复性好，为川芎茶调散的质量研究提供了更加全面的方法。

Objective To develop an HPLC-CAD method for simultaneous determination of chlorogenic acid, ferulic acid, coniferly ferulate, senkyunolide A, Z-ligustilide, imperatorin, and isoimperatorin in Chuanxiong Chatiao Powder. Methods Chromatographic separation was performed on a Capcell Pak MGⅡC₁₈ (250 mm×4.6 mm, 5 μm) column with 80% methanol - 10 mmol/L ammonium formate as the mobile phase for gradient elution. The flow rate was 1.0 mL/min, and the column temperature was maintained at 40 ℃. The nebulization temperature of detector was 35 ℃, the acquisition frequency was 10 Hz, and the filtration constant was 5.0. Injection volume was 20 μL, and diluent was used as 70% methanol. Results Chlorogenic acid, ferulic acid, coniferly ferulate, senkyunolide A, Z-ligustilide, imperatorin, and isoimperatorin had good linear in the ranges of 0.51 — 10.20 μg/mL (r = 0.999 4), 0.53 — 10.60 μg/mL (r = 0.999 3), 2.52 — 50.40 μg/mL (r = 0.999 5), 5.05 — 101.00 μg/mL (r = 0.999 4), 5.01 — 100.20 μg/mL (r= 0.999 8), 1.02 — 20.40 μg/mL (r = 0.999 5), and 0.62 — 12.40 μg/mL (r =0.9993), respectively. The average recoveries were 99.46%, 98.65%, 98.48%, 98.74%, 98.60%, 98.69%, and 98.58% with RSD values of 1.62%, 0.87%, 1.03%, 1.20%, 1.25%, 0.69%, and 0.66%, respectively. Conclusion The method is accurate and reproducible to provide a more comprehensive method for the quality control of Chuanxiong Chatiao Powder.

R286.02