目的 选择合适的中和剂消除增效复合胺消毒剂的抑菌作用，避免样品中微生物限度检查造成假阴性。方法 参照《中国药典》2020年版四部微生物限度有关规定，联合薄膜过滤法，分别采用3种中和剂配方去除季铵盐类消毒剂抗菌活性。结果 采用组合配方中和剂（聚山梨酯、皂苷、卵磷脂、组氨酸比例30：30：3：1）并联合薄膜过滤法可消除季铵盐类消毒剂中的抑菌活性。结论 该方法所用中和剂组方经济易得，可为季铵盐类消毒剂微生物限度检查方法的建立提供依据。

Objective To select the appropriate neutralizing agent to eliminate the bacteriostatic effect of synergistic serotonin disinfectant and avoid false negative caused by microbial limit test in the sample. Methods Three neutralizer formulation were used to remove the antibacterial activity of quaternary ammonium salt disinfectant according to the applicability test of microbial limit count method provide by Chinese Pharmacopoeia (2020 Edition) volume Ⅳ and combined with the filtration and verification method. Results The method by using neutralizer formulation (Tween, saponin, lecithin, and histidine with proportion of 30:30:3:1) and filtration and verification method could eliminate the antibacterial activity of quaternary ammonium salt disinfectant. Conclusion The neutralizer formulation is economically and easily available, which can provide a basis for the establishment of microbial limit test method for quaternary ammonium salt disinfectants.

R943