目的 采用网络药理学方法，科学阐释肾康注射液治疗慢性肾功能衰竭（CRF）的作用机制。方法 通过TCMSP数据库获取肾康注射液的活性成分、潜在药物靶点，通过OMIM、TTD、GeneCards数据库获得CRF的相关靶点，并结合相关软件进行网络药理学分析，包括蛋白质-蛋白质相互作用（PPI）、基因本体（GO）分析、京都基因和基因组百科全书（KEGG）分析。结果 从TCMSP数据库共筛选得到肾康注射液活性成分96个，包括槲皮素、山柰酚、木犀草素等核心成分，涉及相关基因靶点244个。PPI网络涉及93个药物靶点，度值排名前10的核心靶点分别是AKT1、VEGFA、IL6、TNF、TP53、MAPK1、CASP3、EGF、MMP9、EGFR。GO功能分析共获得710个条目（P<0.05），KEGG通路富集得到112条信号通路（P<0.05），包括PI3K-Akt、TNF、HIF-1等信号通路。结论 肾康注射液治疗CRF是多成分、多靶点、多通路的综合效应。

Objective Using network pharmacology, scientifically explain the mechanism of Shenkang Injection in the treatment of chronic renal failure (CRF). Methods Active ingredients and potential drug targets of Shenkang Injection were obtained through TCMSP database, CRF related targets were searched through OMIM, TTD, GeneCards databases, and perform network pharmacological was analyzed by related software, including protein-protein interaction (PPI), Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Results A total of 96 active ingredients of Shenkang Injection were screened from the TCMSP database, including core ingredients such as quercetin, kaempferol, luteolin, and 244 related gene targets. The PPI network involves 93 potential drug targets, and the top 10 core targets were AKT1, VEGFA, IL6, TNF, TP53, MAPK1, CASP3, EGF, MMP9, and EGFR. GO function analysis obtained a total of 710 entries (P<0.05), 112 signal pathways of KEGG pathway enrichment were obtained (P<0.05), including PI3K-Akt, TNF, HIF-1 and other signal pathways. Conclusion The treatment of CRF with Shenkang Injection is a comprehensive effect of multiple components, multiple targets and multiple pathways.

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国家自然科学基金青年科学基金资助项目（81900468）；河南省医学科技攻关计划联合共建项目（LHGJ20190273）