目的 研究促肝细胞生长素对异烟肼和利福平诱导大鼠肝损伤的保护作用。方法 雄性SD大鼠给予异烟肼50 mg/kg+利福平100 mg/kg，建立肝损伤模型。促肝细胞生长素组在模型组的基础上给予促肝细胞生长素肠溶胶囊40 mg/kg，给药3周后，称体质量，计算肝系数，同时比较血清肝功能指标丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、碱性磷酸酶（ALP）、总胆红素（TBIL）和直接胆红素（DBIL），肝抗氧化酶系超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、还原型谷胱甘肽（GSH）和丙二醛（MDA）水平，切片观察肝脏病理变化。结果 与模型组相比，促肝细胞生长素组ALT、AST显著降低（P<0.05），ALP、TBIL和DBIL水平也表现出明显的降低趋势；肝组织SOD、GSH-Px水平显著升高（P<0.05），MDA水平显著降低（P<0.05），GSH水平有升高趋势。促肝细胞生长素组肝小叶结构清晰，炎症细胞浸润减少。结论 促肝细胞生长素对异烟肼和利福平诱导肝损伤具有保护作用，其作用机制可能与其肝再生增强因子提高肝组织抗氧化能力、抑制肝组织炎症反应有关。

Objective To study the protective effects of hepatocyte growth-promoting factors on liver injury induced by isoniazid and rifampicin in rats. Methods SD rats were given isoniazid 50 mg/kg + rifampicin 100 mg/kg to establish the model. The hepatocyte growth-promoting factors group was given Hepatocyte Growth-Promoting Factors Enteric-Coated Capsules 40 mg/kg on the basis of the model group. After 3 weeks' intervention, body mass was weighed and liver coefficient was calculated. And the serum liver function indexes ALT, AST, ALP, TBIL, and DBIL, and liver antioxidant enzyme system SOD, GSH-Px, GSH, and MDA were compared, the changes of hepatic pathology was observed by pathological section. Results Compared with the model group, ALT and AST in the hepatocyte growth-promoting factors group were significantly decreased (P<0.05), and ALP, TBIL, and DBIL levels also had the trend of decrease. SOD and GSH-Px levels in the hepatocyte growth-promoting factors group were significantly increased (P<0.05), but MDA level was significantly decreased (P<0.05). GSH level had the trend of increase. The structure of hepatic lobules was clear and the infiltration of inflammatory cells was reduced in the hepatocyte growth-promoting factors group. Conclusion Hepatocyte growth-promoting factors have the protective effects of liver injury induced by isoniazid and rifampicin in rats, and its mechanism may be related to the enhancement of antioxidant capacity of liver tissue and the inhibition of inflammatory response of liver tissue.

R966

浙江省2018年度重点研发计划项目（2018C02051）