目的 研究芹菜素与γ-环糊精（γ-CD）包合物的制备、表征、包合机制及其抗氧化活性。方法 采用加热回流共沉淀法制备芹菜素γ-环糊精包合物，红外光谱法（IR）对形成的包合物进行表征，荧光光谱法测定包合物的包合常数（K）和包合比（n）；荧光光谱法和核磁共振法（NMR）研究芹菜素与γ-CD在溶液中形成的包合物；双倒数曲线法研究包合前后对自由基1，1-二苯基-2-三硝基苯肼（DPPH·）的清除率。结果 在不同的pH值溶液中，γ-CD对芹菜素具有不同的包合能力，γ-CD最适合在中性介质中与芹菜素形成包合物，在实验浓度范围内按1：1形成包合物，包合比为1 236，包合物对自由基DPPH·的清除活性更强；NMR确定包合物的结构。结论 在实验条件下，芹菜素分子从γ-CD大口端进入到空腔内形成稳定的包合物，且抗氧化活性增加。

Objective To study the preparation, structure, inclusion mechanism and antioxidant activity of apigenin γ-cyclodextrin (γ-CD) inclusion complex. Methods Apigenin γ-cyclodextrin inclusion complex was prepared by heating reflux coprecipitation method. The inclusion complex was characterized by infrared spectroscopy (IR). The inclusion constant (K) and inclusion ratio (n) of the inclusion complex were determined. The inclusion complex of apigenin and γ-CD in solution was studied by fluorescence spectroscopy and nuclear magnetic resonance (NMR). The scavenging rate of 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH·) before and after inclusion was studied by double reciprocal curve method. Results In different pH solutions, γ-CD has different inclusion ability to apigenin. γ-CD was most suitable to form inclusion complex with apigenin in neutral medium. In the range of experimental concentration, apigenin and γ-CD form inclusion complex was at 1:1, the inclusion ratio was 1 236, and apigenin/γ-CD inclusion complex was more stronger in scavenges free radical DPPH· than free apigenin. The structure of the inclusion complex was confirmed by NMR. Conclusion Under the experimental conditions, apigenin molecule is encapsulated into the cavity from the large mouth of γ-CD, forming a stable inclusion complex, and the antioxidant activity is increased.

R944

山西省应用基础青年研究计划项目（201901D211429）；大同市应用基础研究计划项目（2019170）