目的 观察蜂毒注射液对人类风湿关节炎成纤维样滑膜细胞（类风湿关节炎-FLS）B淋巴细胞瘤-2基因（Bcl-2）、Bcl-2相关X蛋白（Bax）mRNA和蛋白的影响，探讨蜂毒注射液治疗类风湿关节炎的作用机制。方法 滑膜组织取自行关节置换或关节镜手术的4名活动期类风湿关节炎患者，分离出FLS并原代培养，逆转录-聚合酶链反应（RT-PCR）观察Bax、Bcl-2 mRNA表达的变化，酶联免疫吸附测定（ELISA）法检测Bax、Bcl-2蛋白水平的变化。结果 与对照组比较，蜂毒注射液4、8、16 mg/L组能促进Bax mRNA表达（P<0.05），蜂毒注射液8、16 mg/L组抑制Bcl-2 mRNA表达（P<0.05、0.01）；蜂毒注射液4、8、16 mg/L组提高Bax蛋白水平，降低Bcl-2蛋白水平（P<0.05、0.01），且有一定的剂量相关性。结论 蜂毒注射液可能通过抑制Bcl-2蛋白、促进Bax蛋白的表达，达到促进类风湿关节炎-FLS凋亡的作用。

Objective To observe the effect of Apistoxin Injection on B-lymphoma-2 gene (Bcl-2), Bcl-2-related X protein (Bax) mRNA and protein in human rheumatoid arthritis fibroblast like synoviocytes (RA-FLS), and to explore the mechanism of Apistoxin Injection in treatment of rheumatoid arthritis. Methods FLS was isolated from synovium of 4 active RA patients who underwent arthroscopic surgery or joint replacement. The expression of Bax and Bcl-2 mRNA was observed by Real time-PCR (RT-PCR) method, and the contents of Bax and Bcl-2 protein were detected by ELISA method. Results Compared with the control group, Apistoxin Injection 4, 8, and 16 mg/L groups could promote the expression of Bax mRNA (P < 0.05), and Apistoxin Injection 8 and 16 mg/L groups could inhibit the expression of Bcl-2 mRNA (P < 0.05, 0.01). Apistoxin Injection 4, 8, and 16 mg/L groups could increase the content of Bax protein and decrease the content of Bcl-2 protein (P < 0.05, 0.01), which was dose-dependent. Conclusion Apistoxin injection may promote the apoptosis of RA-FLS by inhibiting the expression of Bcl-2 protein and promoting the expression of Bax protein.

R966

深圳市南山区技术研发和创意设计项目（2014046）