目的 探究复方景川片对大鼠缺血性脑损伤的保护作用并探讨其对低氧诱导因子-1α（HIF-1α）表达的影响。方法 60只大鼠按体质量随机分为假手术组、模型组、尼莫地平（0.012 g/kg）组和复方景川片（0.780、1.560、3.120 g/kg）组，除假手术组外，其余各组均采用线栓法构建大鼠大脑中动脉闭塞模型，连续给药10 d。采用评分法测定复方景川片对大鼠神经行为学的影响，采用TTC染色法测定脑梗死比例，采用TUNEL染色法检测大鼠缺血区脑组织神经细胞凋亡比率，并通过Real-Time PCR法和Western blotting法检测复方景川片对HIF-1α mRNA和蛋白表达水平的影响。结果 与模型组比较，复方景川片各剂量组均能降低大鼠神经功能评分和横木行走实验评分，能显著性减少神经细胞凋亡、减小梗死体积，其中0.78、1.56、3.12 g/kg组脑梗死面积分别为44.53%、33.71%、29.93%。HIF-1α在复方景川片各剂量组中呈高表达状态，其升高水平与模型组相比具有统计学意义（P<0.05、0.01）。结论 复方景川片在一定剂量范围内能够有效改善大鼠脑缺血损伤程度并减少神经细胞凋亡，其对脑缺血损伤中的神经保护作用可能与其对HIF-1α表达的调控有关。

Objective To explore the protective effect of Compound Jingchuan Tablets on cerebral ischemic injury model in rats and its effect on the expression of hypoxia-inducible factor-1α (HIF-1α). Methods Totally sixty rats were randomly divided into Sham group, model group, nimodipine (0.012 g/kg) group, and Compound Jingchuan Tablets (0.78, 1.56, and 3.12 g/kg) group according body weights. Except Sham group, the middle cerebral artery occlusion model of rats was established with thread blocking method in all groups for 10 d. The effects of Compound Jingchuan Tablets on neurobehaviour in rats were determined by scoring method, the proportion of cerebral infarction was determined by TTC staining, the percentage of neuronal apoptosis in ischemic brain tissue was detected by TUNEL staining, and the expression of HIF-1α was detected by Real-Time PCR and Western blotting methods. Results Compared with the model group, each dose group of Compound Jingchuan Tablets could decrease the neurological function score and the horizontal wood walking test score of rats, and significantly reduce neuronal apoptosis and infarct volume. The infarct area of 0.78, 1.56, and 3.12 g/kg groups were 44.53%, 33.71%, and 29.93%, respectively. It was highly expressed in the various dose groups of Compound Jingchuan Tablets, and its elevated level was statistically significant compared with the model group (P<0.05 or P<0.01). Conclusion Compound Jingchuan Tablets can effectively improve the degree of cerebral ischemia injury and reduce neuronal apoptosis in rats within a certain dose range. Its neuroprotective effect on cerebral ischemia injury may be related to its regulation of HIF-1a expression.

天津市自然科学基金资助项目（17JCYBJC28700）；天津市卫生和计划生育委员会中医中西医结合科研课题（2017079）；天津市卫生健康委、天津市中医药管理局中医、中西医结合科研课题（2019105）