目的 研究迷迭香酸通过线粒体通路诱导骨肉瘤MG-63细胞凋亡的作用。方法 体外培养MG-63细胞，将10、20、30、40、50、60 μmol/L迷迭香酸作用于MG-63细胞，MTT和CCK-8法检测迷迭香酸对细胞生长的影响；AO/EB染色法观察细胞凋亡；流式细胞仪检测对细胞凋亡和细胞周期的影响；Western blotting法检测Cyt-c、Caspase-3、Bax和Bcl-2蛋白表达水平。结果 MTT和CCK-8结果显示迷迭香酸可浓度相关性地抑制MG-63细胞的生长；AO/BE染色可看到迷迭香酸组凋亡细胞明显增加；流式细胞仪检测结果显示迷迭香酸可浓度相关性地促进MG-63细胞凋亡，并将细胞周期阻滞在S期；Western blotting法结果显示与对照组比较，迷迭香酸20、40、60 μmol/L组的Bax、Caspase-3和Cyt-c蛋白表达水平明显增加（P<0.05、0.01）；与对照组比较，迷迭香酸20、40、60 μmol/L组的Bcl-2蛋白表达水平则明显降低（P<0.05、0.01），且呈剂量相关性。结论 迷迭香酸可显著促进人骨肉瘤MG-63细胞凋亡，其机制可能与调控线粒体凋亡通路有关。

Objective To investigate the effects of rosanoic acid on apoptosis of human osteosarcoma cell line MG-63 in vitro through mitochondrial pathway. Methods MG-63 cells were cultured in vitro, and MTT and CCK-8 were performed to observe the proliferation after treated with rosanoic acid 10, 20, 30, 40, 50, and 60 μmol/L. AO/EB staining was used to detect the porphological changes of apoptotic cells. Flow cytometry was used to determine the apoptosis rate and cell cycle. Cyt-c, Caspase-3, Bax, and Bcl-2 contents were detected by Western blotting. Results MTT and CCK-8 results showed that rosanoic acid could inhibit the proliferation of MG-63 cells by concentration-dependent manner. The number of apoptosis cells in rosanoic acid groups was increased after AO/EB staining significantly. Flow cytometry showed that rosanoic acid could promote the apoptosis of MG-63 cells in a concentration-dependent manner and the cell cycle was arrest at S phase. Western blotting results showed that compared with the control group, the levels of Bax, Caspase-3, and Cyt-c in the rosanoic acid 20, 40, and 60 μmol/L groups were increased significantly (P<0.05, 0.01), but the levels of Bcl-2 in the rosanoic acid 20, 40, and 60 μmol/L groups were decreased significantly (P<0.05, 0.01), and they had the dose-dependence. Conclusion Rosanoic acid can promote the apoptosis of human osteosarcoma MG-63 cells significantly, and its mechanism may be related to the regulation of mitochondrial apoptosis pathway.