目的 研究胃肠安联合替加氟对结肠癌HT29细胞的体内外抑制作用。方法 采用MTT法测定胃肠安联合替加氟对结肠癌HT29细胞的抑制作用，并采用流式细胞仪检测结肠癌HT29细胞中CD133⁺的表达情况。建立结肠癌HT29细胞裸鼠模型，对照组尾静脉注射生理盐水，0.2 mL/次，2次/周。替加氟组尾iv替加氟注射液，36 mg/kg，1次/周；胃肠安丸组，用纯净水将按胃肠安丸配成4 mg/kg，ig 0.2 mL/d；胃肠安+替加氟组尾iv替加氟注射液，36 mg/kg，1次/周；同时，用纯净水将按胃肠安丸配成4 mg/kg，ig 0.2 mL/d。观察胃肠安联合替加氟对荷瘤裸鼠生存状况和结肠癌HT-29细胞的影响。结果 与对照组比较，替加氟组、胃肠安组、胃肠安+替加氟组抑制结肠癌HT-29细胞的增殖能力明升高，差异有统计学意义（P<0.05）；胃肠安+替加氟组明显高于单独使用替加氟组、胃肠安组，差异有统计学意义（P<0.05）。与对照组比较，替加氟组、胃肠安组、胃肠安+替加氟组的CD133⁺细胞比例明显降低，差异有统计学意义（P<0.05）；胃肠安+替加氟组明显低于单独使用替加氟组、胃肠安组，差异有统计学意义（P<0.05）。治疗后，胃肠安组、替加氟组、胃肠安+替加氟组的实体瘤质量明显低于对照组，差异具有统计学意义（P<0.05）；胃肠安+替加氟组的实体瘤质量明显低于胃肠安组和替加氟组，差异有统计学意义（P<0.05）。结论 胃肠安联合替加氟对结肠癌HT29细胞及其干细胞的增殖有抑制作用。

Objective To study the inhibitory effects Weichang'an combined with tegafur on colon cancer HT29 cells in vitro and in vivo. Methods The inhibition of Weichang'an combined with tegafur on colon cancer HT29 cells was detected by MTT method. The expression of CD133⁺ was detected by flow cytometry. The nude mouse model of colon cancer HT29 cells was established. Rats in the control group were given normal saline, 0.2 mL/time, twice per week. Tegafur Injection was given by tail vein injection, 36 mg/kg, once per week. Purified water was added into Weichang'an Pills to 4 mg/kg, ig 0.2 mL/d. Rats in Weichang'an+tegafur group were given Tegafur Injection by tail vein injection, 36 mg/kg, once per week. Purified water was added into Weichang'an Pills to 4 mg/kg, ig 0.2 mL/d. The effects of Weichang'an combined with tegafur on living condition of nude mice bearing tumor and colon cancer HT29 cells were observed. Results Compared with the control group, the inhibition of Weichang'an combined with tegafur on colon cancer HT29 cells in tegafur group, Weichang'an group, and Weichang'an+tegafur group were significantly increased (P<0.05). The inhibition in Weichang'an+tegafur group was higher than that in tegafur group and Weichang'an group (P<0.05). Compared with the control group, the proportion of CD133⁺cell in tegafur group, Weichang'an group, and Weichang'an+tegafur group were significantly decreased (P<0.05). The proportion in Weichang'an+tegafur group was lower than that in tegafur group and Weichang'an group (P<0.05). After treatment, solid tumor mass in tegafur group, Weichang'an group, and Weichang'an+tegafur group were lower than that in the control group (P<0.05). The solid tumor mass in Weichang'an+tegafur group was lower than that in tegafur group and Weichang'an group (P<0.05).Conclusion Weichang'an combined with tegafur have the inhibition of proliferation of colon cancer HT29 cells and their stem cells.

天津市卫生健康委、天津市中医药管理局中医、中西医结合科研课题（2019035）