目的 建立HPLC波长切换法测定益坤宁酊中梓醇、麦角甾苷、马替诺皂苷、氧化芍药苷、芍药内酯苷、芍药苷和α-香附酮的方法。方法 采用Agilent Eclipse XDB-C₁₈色谱柱（250 mm×4.6 mm，5μm）；流动相A：乙腈–甲醇（1∶3），流动相B：0.2%磷酸溶液，梯度洗脱；检测波长210 nm（梓醇）、330 nm（麦角甾苷、马替诺皂苷）和230 nm（氧化芍药苷、芍药内酯苷、芍药苷、α-香附酮）；体积流量0.8 mL/min；柱温25℃；进样量10 μL。结果 梓醇、麦角甾苷、马替诺皂苷、氧化芍药苷、芍药内酯苷、芍药苷和α-香附酮在2.66～66.50、1.59～39.75、0.76～19.00、1.38～34.50、7.02～175.50、9.91～247.75、1.19～29.75 μg/mL线性关系良好。平均加样回收率分别为97.97%、98.31%、96.99%、97.78%、99.28%、100.03%、97.43%，RSD值分别为1.42%、0.92%、1.30%、1.13%、1.08%、0.65%、1.27%。结论 本法操作便捷、数据准确、灵敏度高，重复性好，可为益坤宁酊的质量控制提供参考。

Objective To develop an HPLC with wavelength switching method for determination of catalpol, acteoside, martinoside, oxypaeoniflorin, albiflorin, paeoniflorin and α-cyperone in Yikunning Tincture. Methods The separation was performed on Agilent Eclipse XDB-C₁₈ column (250 mm×4.6 mm, 5 μm). The mobile phase consisted of mobile phase A acetonitrile-methanol (1:3) and mobile phase B 0.2% phosphoric acid solution with gradient elution. The detection wavelengths were set at 210 nm (catalpol), 330 nm (acteoside and martinoside), and 230 nm (oxypaeoniflorin, albiflorin, paeoniflorin, and α-cyperone). The flow rate was 0.8 mL/min, temperature of column was set at 25℃, and volume of injection was 10 μL. Results The linear ranges of catalpol, acteoside, martinoside, oxypaeoniflorin, albiflorin, paeoniflorin, and α-cyperone were 2.66-66.50, 1.59-39.75, 0.76-19.00, 1.38-34.50, 7.02-175.50, 9.91-247.75, and 1.19-29.75 μg/mL, respectively. The average recoveries were 97.97%, 98.31%, 96.99%, 97.78%, 99.28%, 100.03%, and 97.43% with RSD 1.42%, 0.92%, 1.30%, 1.13%, 1.08%, 0.65%, and 1.27%, respectively. Conclusion The method is convenient, accurate, sensitive, and reproducible, which can provide reference for quality control of Yikunning Tincture.

广东省中医药强省项目（20142167）