目的 考察白藜芦醇对烟熏和脂多糖诱导大鼠慢性阻塞性肺疾病的改善作用，探讨其Derlin-1蛋白调控的作用机制。方法 通过烟熏和滴注脂多糖的方法建立大鼠慢性阻塞性肺疾病模型。分别每日ig 15、45 mg/kg白藜芦醇，28 d后采用Tunel法检测肺泡上皮细胞凋亡情况，ELISA法检测肺促炎细胞因子水平，免疫组化和Western blotting法评价Derlin-1、JNK和磷酸化JNK（p-JNK）的表达水平。结果 与模型组比较，45 mg/kg白藜芦醇能够改善大鼠肺泡上皮细胞凋亡，显著降低促炎细胞因子、Derlin-1和JNK的磷酸化水平。结论 白藜芦醇能够改善慢性阻塞性肺疾病大鼠的肺损伤，其作用机制与调控肺Derlin-1蛋白有关。

Objective To study the improvement of resveratrol on chronic obstructive pulmonary disease (COPD) rat induced by smoking and lipopolysaccharide, and explore its mechanism of regulation of lung Derlin-1 protein. Methods Rat models of COPD were induced by smoking and lipopolysaccharide instillation. Rats were ig administered with resveratrol of 15 or 45 mg/kg for 28 d. Then, the apoptosis was evaluated by Tunel staining. The levels of proinflammatory cytokines were determined by ELISA method. The expressions of Derlin-1, JNK, and phosphorylation (p)-JNK were detected by immunohistochemistry and Western blotting method. Results Compared with the model group, resveratrol (45 mg/kg) could improve the apoptosis of alveolar epithelial cells, and significantly reduce the levels of proinflammatory cytokines, Derlin-1, and JNK phosphorylation. Conclusion Resveratrol can improve the pulmonary injury in COPD rats and its mechanism is related to the regulation of lung Derlin-1 protein.

武汉市卫生计生科研基金项目（WZ16C20，WX16B11）