[关键词]
[摘要]
目的 通过体外抗氧化活性检测,比较密蒙花与其替代品结香总提物体外抗氧化能力的差异。方法 采用福林酚法测定密蒙花、结香总提物中总酚,并采用DPPH自由基清除能力法、ABTS清除能力法、Fe3+还原能力法和Fe2+螯合能力法分别测定两者体外抗氧化能力,选取维生素C和EDTA-Na2作为阳性对照。结果 密蒙花中总酚为结香的3~5倍;除亚铁离子螯合试验外,密蒙花的抗氧化作用均明显优于结香,这与总酚的量呈正相关性。结论 两者总提取物中总酚和抗氧化能力差异显著,密蒙花明显优于结香。
[Key word]
[Abstract]
objective To compare the antioxidant activity in vitro of total extraction for Buddleja officinalis Maxim. and its substitute Edgeworthia chrysantha Lindl. Methods The contents of total phenolic were determined by Folin-Ciocalteu method. Antioxidant abilities of B. officinalis and E. chrysantha in vitro were determined using the method of DPPH free radical clear ability, ABTSclear ability, Fe3+ reduction ability, and Fe2+ chelating capacity, and VC and EDTA-Na2 were selected as positive control. Results The total phenolic content of the B. officinalis was about 3-5 times that of the E. chrysantha. Except for Fe2+ chelation test, the antioxidant effect of the B. officinalis was clearly superior to E. chrysantha, which was positively correlated with the contents of the total phenolic. Conclusion The total phenolic content and antioxidant capacity of both are significant different, and B. officinalis is better than that of E. chrysantha.
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[基金项目]
国家自然科学基金资助项目(81503220);江苏省自然科学基金资助项目(BK20150706)