目的 建立HPLC梯度洗脱法测定复方鹿茸酒中尿囊素、山药素I、淫羊藿苷、淫羊藿次苷I和宝藿苷I。方法 采用Agilent TC-C₁₈色谱柱（250 mm×4.6 mm，5 μm）；流动相A：甲醇-乙腈（1：1）、流动相B：水，梯度洗脱；0~22 min在224 nm波长下检测尿囊素，22~45 min时在270 nm波长下检测山药素Ⅰ、淫羊藿苷、淫羊藿次苷Ⅰ和宝藿苷Ⅰ；体积流量0.9 mL/min；柱温30℃；进样量10 μL。结果 尿囊素、山药素I、淫羊藿苷、淫羊藿次苷I和宝藿苷I分别在7.98~159.60 μg/mL、4.75~95.00μg/mL、9.18~183.60 μg/mL、4.54~90.80μg/mL、4.92~98.40 μg/mL与峰面积线性关系良好；平均回收率分别为98.67%、96.86%、99.56%、98.15%、97.90%，RSD值分别为1.12%、0.85%、1.07%、1.49%、0.97%。结论 该方法简便、准确，重复性好，为进一步完善复方鹿茸酒的质量标准提供参考。

Objective To develop an HPLC gradient elution method for determination of allantoin, batatasin I, icariin, icarisid I and baohuoside I in Compound Lurong Vina. Methods HPLC method was adopted on Agilent TC-C₁₈ column (250 mm×4.6 mm, 5 μm). The mobile phase consisted of methanol-acetonitrile (1:1)-water with gradient elution. The detection wavelengths were 224 nm in 0-22 min (determination of allantoin) and 270 nm in 22-45 min (determination of icariin, icarisid I, and baohuosideⅠ). The flow rate was 0.9 mL/min, and the column temperature was set at 30℃ with injection volume of 10 μL. Results Allantoin, batatasin I, icariin, icarisid I, and baohuoside I had good linearity in the ranges of 7.98-159.60 μg/mL, 4.75-95.00 μg/mL, 9.18-183.60 μg/mL¹, 4.54-90.80 μg/mL, and 4.92-98.40 μg/mL, respectively. The average recoveries were 98.67%, 96.86%, 99.56%, 98.15%, and 97.90%, with RSD of 1.12%, 0.85%, 1.07%, 1.49%, and 0.97%, respectively. Conclusions The method is simple, accurate and reproducible, and can provide a reference for the further improvement of the quality standard of Compound Lurong Vina.