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[摘要]
目的 建立同时测定复方双花片中绿原酸、咖啡酸、连翘苷、穿心莲内酯和木犀草素的方法。方法 采用Agilent 5 TC-C18色谱柱(150 mm×4.6 mm,5 μm),流动相:乙腈–0.4%磷酸溶液,梯度洗脱;检测波长切换0~16 min(327 nm)、16~30 min(228 nm);体积流量:1.0 mL/min;柱温:30 ℃,进样量:10 μL。结果 绿原酸、咖啡酸、连翘苷、穿心莲内酯和木犀草素在161.6~1616.0 ng(r=1.000 0)、12.48~124.80 ng(r=0.999 9)、14.30~143.04 ng(r=0.999 8)、13.48~134.80 ng(r=1.000 0)、8.72~87.20 ng(r=1.000 0)线性关系良好;平均回收率分别为101.15%、98.72%、101.42%、99.15%、98.76%,RSD值分别为1.83%、2.58%、1.69%、1.20%、1.96%。结论 该方法操作简单,专属性强,为更好地评价复方双花片质量提供了参考。
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[Abstract]
Objective To develop a method for determination of chlorogenic acid, caffeic acid, forsythin, andrographalide, and luteolin in Compound Shuanghua Tablets by HPLC.Methods The separation was performed on Agilent 5TC-C18 column (150 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile -0.4% phosphoric acid with gradient elution. The detection wavelengths were set at 327 nm in 0-16 min and 228 nm in 16-30 min. The flow rate was 1.0 mL/min, temperature of column was set at 30 ℃, and volume of injection was 10 μL. Results The linear range of chlorogenic acid, caffeic acid, forsythin, andrographalide, and luteolin were 161.6-1616.0 ng (r=1.000 0), 12.48-124.80 ng (r=0.999 9), 14.30-143.04 ng (r=0.999 8), 13.48-134.80 ng (r=1.000 0), 8.72-87.20 ng (r=1.000 0), respectively. The average recoveries were 101.15%, 98.72%, 101.42%, 99.15%, and 98.76% with RSD 1.83%, 2.58%, 1.69%, 1.20%, and 1.96%, respectively. Conclusion The method is simple and specific, and provides a reference for quality of Compound Shuanghua Tablets.
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