目的 建立HPLC波长切换联合梯度洗脱法同时测定调中四消丸中咖啡酸、芦荟大黄素、大黄酸、大黄素甲醚、圆柚酮、α-香附酮。方法 采用Agilent TC-C₁₈色谱柱（250 mm×4.6 mm，5 μm）；流动相：乙腈–0.1%磷酸溶液，梯度洗脱；0～21 min在325 nm波长下检测咖啡酸；21～37 min在254 nm波长下检测芦荟大黄素、大黄酸和大黄素甲醚，37～55 min在242 nm波长下检测圆柚酮和α-香附酮；体积流量：0.9 mL/min；柱温：30 ℃；进样量为10 μL。结果 咖啡酸、芦荟大黄素、大黄酸、大黄素甲醚、圆柚酮、α-香附酮分别在2.48～49.60、17.56～351.20、4.59～91.80、6.76～135.20、1.92～38.40、4.26～85.20 μg/mL与峰面积线性关系良好；平均回收率分别为98.01%、99.27%、97.61%、99.41%、96.90%、98.58%，RSD值分别为0.77%、1.18%、0.96%、1.25%、1.48%、1.17%（n = 6）。结论 本方法简单快速，可作为调中四消丸的质量控制方法。

Objective To establish an HPLC wavelength switching combined gradient elution method for simultaneous determination of six major components, including caffeic acid, aloe-emodin, rhein, physcion, nootkatone and α-cyperone in Tiaozhong Sixiao Pills. Methods The determination was carried out on Agilent TC-C₁₈ column (250 mm × 4.6 mm, 5 μm). The mobile phase was consisted of acetonitrile -0.05% phosphoric acid solution with gradient elution. The detection wavelengths were 325 nm (for caffeic acid), 254 nm (for aloe-emodin, rhein, and physcion), and 242 nm (for nootkatone and α-cyperone). The column temperature was set at 30 ℃, and the flow rate was 0.9 mL/min with injection volume of 10 μL. Results There were good linear relationships of caffeic acid, aloe-emodin, rhein, physcion, nootkatone, and α-cyperone in the concentration ranges of 2.48-49.60, 17.56-351.20, 4.59-91.80, 6.76-135.20, 1.92-38.40, and 4.26-85.20 μg/mL, respectively. The average recoveries were 98.01%, 99.27%, 97.61%, 99.41%, 96.90%, and 98.58% with RSD of 0.77%, 1.18%, 0.96%, 1.25%, 1.48%, and 1.17% (n=6), respectively. Conclusion The method is simple and rapid, which can be used as quality control methods for Tiaozhong Sixiao Pills.