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[摘要]
目的 探讨槲皮素对人胃癌MKN45细胞凋亡的影响及其作用机制。方法 取对数生长期的MKN45细胞分为对照组和20、40、80 μmol/L槲皮素组。应用MTT比色法测定细胞活性,Hoechst染色、流式细胞技术检测细胞凋亡,比色法检测凋亡相关因子Caspase-3、Caspase-9表达。结果 槲皮素能显著抑制MKN45细胞增殖,促进细胞凋亡,升高Caspase-3、Caspase-9基因表达水平。结论 槲皮素可通过增强Caspase-3、Caspase-9基因表达诱导人胃癌MKN45细胞凋亡。
[Key word]
[Abstract]
Objective To investigate the effect and mechanism of quercetin on MKN45 cells. Methods MKN45 cells were divided into four group: negative control group and quercetin groups (20, 40, and 80 μmol/L). Cell viability was measured by MTT colorimetric assay, apoptosis was detected by Hoechst staining and flow cytometry assay, and expression of apoptosis-related factors Caspase-3 and Caspase-9 were tested by colorimetric detection. Results Quercetin could obviously inhibit MKN45 cell proliferation and promote apoptosis, and could increase Caspase-3 and Caspase-9 gene expression levels. Conclusion Quercetin can induce gastric carcinoma MKN45 cells apoptosis by enhancing the expression of Caspase-3 and Caspase-9 gene.
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[基金项目]
天津市中医中西医结合科研课题(2015054)