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[摘要]
目的 采用多种实验模型评价注射用复方骨肽的药效学,并探讨其作用机制。方法 采用实验性大鼠胫骨骨折模型观察注射用复方骨肽的治疗作用;采用小鼠扭体及福尔马林痛模型观察注射用复方骨肽的镇痛作用;采用大鼠足肿和小鼠耳肿模型观察注射用复方骨肽的抗炎作用。结果 与模型组比较,给药2周,注射用复方骨肽15、45 mg/kg组血清转化生长因子β1(TGF-β1)、骨形成蛋白(BMPs)水平明显升高;给药3周骨折程度明显减轻,愈合率明显升高(P< 0.05、0.01)。与对照组比较,注射用复方骨肽30、90 mg/kg组明显减少扭体次数(P< 0.05、0.01);注射用复方骨肽90 mg/kg明显减少舔足时间。与模型组比较,注射用复方骨肽15、45 mg/kg组明显减轻角叉菜胶引起的大鼠足肿胀。与对照组比较,注射用复方骨肽90 mg/kg明显减轻二甲苯引起的耳肿胀。结论 注射用复方骨肽具有明显的镇痛、消炎作用,对实验性骨折有明显的治疗作用,作用机制可能是促进生长因子TGF-β1、BMPs的合成,加快骨折愈合。
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[Abstract]
Objective To evaluate the pharmacodynamics of Compound Ossotide for injection by using various models, and to explore its mechanism. Methods Therapeutic effect of Compound Ossotide for injection was observed by using fracture model of rats tibia induced by hacksaw. Analgesic effect of Compound Ossotide for injection was observed by using mouse twist and faure marin model. Anti-inflammation effect of Compound Ossotide for injection was observed by using the model of rats ankle swelling caused by carrageenin and mice auris swelling caused by dimethylbenzene. Results Compared with model control group treated for two weeks, serum contents of TGF-β1 and BMPs in Compound Ossotide for injection 15 and 45 mg/kg groups were significantly increased. And treated for three weeks, the healing degree of fracture and healing rate were obviously improved (P < 0.05, 0.01). Compared with control group, the number of twisting caused by acetic acid in Compound Ossotide for injection 30 and 90 mg/kg groups were significantly reduced (P < 0.05, 0.01), and the licking foot time after injecting methanol in mice Compound Ossotide for injection 90 mg/kg group was significantly decreased. Compared with model group, Compound Ossotide for injection (90 mg/kg) could obviously reduce the foot swelling induced by carrageenin in rats. Compared with control group, Compound Ossotide for injection (15 and 45 mg/kg) could obviously reduce ear swelling induced by xylene. Conclusion Compound Ossotide for injection have obvious effects of analgesia and anti-inflammatory. The mechanism may be to promote the synthesis of growth factors TGF-β1 and BMPs and accelerate the healing of fracture.
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