目的 建立毛细管电泳法测定（S）-2-（6-羟基-2,3-二氢苯并呋喃-3-基）乙酸甲酯中R异构体的方法。方法 采用毛细管电泳法。以磺酸-β-环糊精（S-β-CD）为选择剂；25 mmol/L硼酸盐缓冲液（pH 8.9，含S-β-CD 1.8%）为运行缓冲液；运行电压为25 kV；柱温为15℃；检测波长：214 nm；3.4 kPa压力进样10 s。结果 （S）-2-（6-羟基-2,3-二氢苯并呋喃-3-基）乙酸甲酯与R异构体的分离度为2.9。R异构体在2~30 μg/mL与峰面积线性关系良好（r=0.999 5），平均回收率为96.6%，RSD值为4.9%（n=6）。结论 建立的毛细管电泳法操作简便，结果准确可靠，可用于（S）-2-（6-羟基-2,3-二氢苯并呋喃-3-基）乙酸甲酯中R异构体的控制。

Objective To establish a capillary electrophoresis method for the determination of R-optical isomer in (S)-methyl-2-(6-hydroxy-2,3-dihydrobenzofuran-3-yl)acetate. Methods Capillary electrophoresis method was adopted. Sulfate-β-cyclodextrin (S-β-CD) was used as selector. The background buffer was 25 mmol/L borate buffer solution (pH 8.9, containing 1.8% S-β-CD). The separation was performed at 15 ℃ with the voltage of 25 kV and the wavelength was 214 nm. Pressure was 3.4 kPa and injected for 10 s. Results (S)-methyl-2-(6-hydroxy-2,3-dihydrobenzofuran-3-yl)acetate and its R-enantiomer were basely resolved with resolution of 2.9. The calibration curve of R-enantiomer was linear in the concentration range of 2 - 30 μg/mL (r = 0.999 5). The average recovery was 96.6% with RSD 4.9% (n = 6). Conclusion The method is convenient, rapid, accurate, and can be used for the determination of (R)-methyl-2-(6-hydroxy-2,3-dihydrobenzofuran-3-yl)acetate in (S)-methyl-2-(6-hydroxy-2,3-dihydrobenzofuran- 3-yl) acetate.

山东省自然科学基金杰出青年基金资助项目（JQ201319）；山东大学自主创新基金资助项目（2016JC018）