目的 建立HPLC波长切换法同时测定醒脾养儿颗粒中的熊果苷、槲皮苷、缬草三酯和乙酰缬草三酯。方法 采用HPLC法，Agilent HC-C₁₈柱色谱柱（250 mm×4.6 mm，5 μm）；流动相：乙腈–0.4%磷酸溶液，梯度洗脱；0~14 min时在280 nm波长下检测熊果苷，14~18 min在350 nm波长下检测槲皮苷，18~30 min在256 nm波长下检测缬草三酯和乙酰缬草三酯；体积流量：0.9 mL/min；柱温：25 ℃；进样量为10 μL。结果 熊果苷、槲皮苷、缬草三酯和乙酰缬草三酯分别在11.97~239.40 μg/mL、4.65~93.00 μg/mL、4.18~83.60 μg/mL、5.30~106.00 μg/mL峰面积与质量浓度呈良好的线性关系，r分别为0.999 8、0.999 3、0.999 9、0.999 5；平均回收率分别为98.78%、97.11%、99.14%、98.75%，RSD值分别为1.62%、0.68%、1.39%、0.87%。结论 所建立的方法样品处理方法简便，检测的准确性和重复性均较好，可为醒脾养儿颗粒质量标准测定方法的制定提供依据。

Objective To develop an HPLC wavelength switching method for simultaneous determination of the contents of arbutin, quercitrin, valepotriate and acevaltrate in Xingpi Yang'er Granules. Methods HPLC method was adopted. The determination was carried out on Agilent HC-C₁₈ chromatographic column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitril - 0.4% phosphoric acid solution with gradient elution. The detection wavelengths were set at 280 nm in 0 — 14 min (determination of arbutin), 350 nm in 14 — 18 min (determination of quercitrin), and 256 nm in 18 — 30 min (determination of valepotriate and acevaltrate). The flow rate was 0.9 mL/min, temperature of column was set at 25 ℃, and volume of injection was 10 μL. Results The linear range ofarbutin, quercitrin, valepotriate and acevaltrate were 11.97 — 239.40 μg/mL (r=0.999 8), 4.650 — 93.00 μg/mL (r=0.999 3), 4.180 — 83.60 μg/mL (r=0.999 9), and 5.300 — 106.0 μg/mL (r=0.999 5), respectively. The average recoveries were 98.78%, 97.11%, 99.14%, and 98.75% with RSD 1.62%, 0.68%, 1.39%, and 0.87%, respectively. Conclusion The established method is simple and accurate, has good repeatability which can be applied to the quality control for Xingpi Yang'er Granules.