目的 比较猪、牛、羊胆汁制胆南星中胆酸类成分及其对发热小鼠体温的影响，为规范胆南星发酵工艺提供依据。方法 分别采用猪、牛、羊胆汁与天南星生品发酵制备胆南星。采用HPLC-CAD法检测3种胆南星中胆酸类成分胆酸、去氧胆酸、猪去氧胆酸、鹅去氧胆酸。以干酵母制备小鼠发热模型，观察猪、牛、羊胆汁制胆南星对发热小鼠体温的影响。结果 不同胆汁制胆南星中胆酸类成分存在差异，牛胆汁制胆南星中胆酸类成分质量分数最高，胆酸、猪去氧胆酸、鹅去氧胆酸、去氧胆酸的质量分数分别为0.035 9%、0.843 4%、2.260 2%、0.037 2%。猪胆汁制胆南星中鹅去氧胆酸和去氧胆酸升高，胆酸和猪去氧胆酸降低；羊胆汁制胆南星中胆酸类成分除去氧胆酸外均降低。不同胆汁制胆南星对发热小鼠均有一定的清热作用，猪胆汁制胆南星组小鼠体温先升高后降低，且与模型组比较差异有显著性，牛胆汁制胆南星亦表现出清热作用，但与模型组比较无显著性差异。羊胆汁制胆南星清热作用较弱。结论 综合比较猪、牛、羊胆汁制胆南星中胆酸类成分质量分数和清热作用，作为制备胆南星的辅料，牛胆汁、猪胆汁优于羊胆汁。

Objective To compare bile acids contents in different Arisaema cum Bile processed with pig's bile, bilis bovina, and sheep bile and discuss the differences of temperature changes in fever mice, and provide the basis for Arisaema cum Bile fermentation process. Methods Arisaema cum Bile were prepared by fermentation on Arisaematis Rhizoma with pig's bile, bilis bovina, and sheep bile. HPLC-CAD method was used to determine the bile acid composition (cholic acid, deoxidationcholic acid, hyodeoxycholic acid, and chenodeoxycholic acid) in three kinds of Arisaema cum Bile. The fever model of mice was established with dry yeast, and the effects of clearing the heat on the models were observed and compared.Results There were significant differences of bile acids among three kinds of Arisaema cum Bile, and bile acids composition contents were the highest in Arisaema cum Bile processed with bilis bovina, and cholic acid, hyodeoxycholic acid, chenodeoxycholic acid, and deoxidationcholic acid were 0.035 9%, 0.843 4%, 2.260 2%, and 0.843 4%, respectively. Contents of chenodeoxycholic acid and deoxidationcholic acid were decreased in Arisaema cum Bile processed with pig's bile, but those of cholic acid and hyodeoxycholic acid were significantly decreased. Contents of bile acids except deoxidationcholic acid in Arisaema cum Bile processed with sheep bile were significantly decreased. The three kinds of Arisaema cum Bile samples all showed clearing heat function. Mice temperature in Arisaema cum Bile processed with pig's bile group increased first and then decreased. Compared with model group, Arisaema cum Bile processed with bilis bovina also showed clearing heat effects, but there was no significant difference. Arisaema cum Bile processed with sheep bile had weak clearing heat function. Conclusion Both the contents of bile acids and the effects of clearing heat are different among Arisaema cum Bile processed with pig's bile, bilis bovina, and sheep bile, and pig's bile and bilis bovina are better than sheep bile as Arisaema cum Bile processing adjuvants.

公益性行业科研专项经费项目（201507004-03）