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[摘要]
目的 建立高效液相色谱法联合二极管阵列检测器(HPLC-DAD)法同时测定野菊花栓中绿原酸、3,5-二咖啡酰奎宁酸、木犀草素-7-O-β-D-葡萄糖苷、芹菜素-7-O-β-D-葡萄糖苷和蒙花苷。方法 采用HPLC-DAD法,Thermo Hypersil GOLD C18色谱柱(250 mm×4.6 mm,5 μm);流动相:0.05%磷酸-乙腈,梯度洗脱;检测波长:327 nm;体积流量:0.8 mL/min;柱温:35℃;进样量:10 μL。结果 绿原酸、3,5-二咖啡酰奎宁酸、木犀草素-7-O-β-D-葡萄糖苷、芹菜素-7-O-β-D-葡萄糖苷和蒙花苷分别在0.105~2.100 μg(r=0.999 9)、0.165~3.300 μg(r=0.999 8)、0.081~1.620 μg(r=0.999 7)、0.060~1.200 μg(r=0.999 6)、0.317~6.340 μg(r=0.999 5)与峰面积呈良好的线性关系,平均加样回收率分别为99.19%、99.54%、99.34%、98.83%和99.26%,RSD值分别为0.87%、1.04%、1.06%、1.29%、0.91%。结论 该方法简便、准确、重复性好,可用于野菊花栓中有机酸和黄酮类多成分的质量控制。
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[Abstract]
Objective To develop an HPLC-DAD method for simultaneous determination of chlorogenic acid, 3,5-dicaffeoylquinic acid, luteolin-7-O-β-D-glucoside, apigenin-7-O-β-D-glucoside, and linarin in Yejuhua Suppository. Methods HPLC-DAD was used. Chromatographic separation was performed on a Thermo Hypersil GOLD C18 (250 mm×4.6 mm, 5 μm), and the mobile phase was acetonitrile-0.05% phosphoric acid with gradient elution. The detection wavelengths were set at 327 nm, flow rate was 0.8 mL/min, the column temperature was maintained at 35℃, and injection volume was 10 μL. Results Chlorogenic acid, 3,5-dicaffeoylquinic acid, luteolin-7-O-β-D-glucoside, apigenin-7-O-β-D-glucoside, and linarin had good linearity in the ranges of 0.105-2.100 μg (r=0.999 9), 0.165-3.300 μg (r=0.999 8), 0.081-1.620 μg (r=0.999 7), 0.060-1.200 μg (r=0.999 6), and 0.317-6.340 μg (r=0.999 5), respectively. The average recoveries were 99.19%, 99.54%, 99.34%, 98.83%, and 99.26%, with RSD of 0.87%, 1.04%, 1.06%, 1.29%, and 0.91%, respectively. Conclusions The method is convenient, accurate and reproducible which can be applied to the quality control of organic acids and flavonoids in Yejuhua Suppository.
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