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[摘要]
目的 建立HPLC法同时测定参茸安神片中远志酮Ⅲ、3,6'-二芥子酰基蔗糖、细叶远志皂苷、远志皂苷B和五味子醇甲的方法。方法 采用Kromasil C18色谱柱(250 mm×4.6 mm,5 μm);流动相:乙腈-0.1%磷酸溶液,梯度洗脱;检测波长:320 nm(0~16 min检测远志酮Ⅲ、3,6'-二芥子酰基蔗糖)、210 nm(16~24 min检测细叶远志皂苷、远志皂苷B)、250 nm(24~35 min检测五味子醇甲);体积流量:1.0 mL/min;柱温:30℃;进样量为10 μL。结果 远志(口山)酮Ⅲ、3,6'-二芥子酰基蔗糖、细叶远志皂苷、远志皂苷B和五味子醇甲分别在3.85~77.00、6.22~124.40、6.57~131.40、7.61~152.20、4.96~99.20 μg/mL线性关系良好(r≥0.999 2);平均回收率分别为98.17%、99.24%、99.90%、97.63%、96.83%,RSD值分别为1.14%、1.23%、0.70%、1.52%、0.81%。结论 本法稳定可靠、简便易行,为全面控制参茸安神片的质量提供了参考。
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[Abstract]
Objective To establish an HPLC method for simultaneous determination of polygalaxanthone Ⅲ, 3,6'-disinapoyl sucrose, tenuifolin, onjisaponin B, and schisandrin in Shenrong Anshen Tablets. Methods The determination was carried out with Kromasil C18 column (250 mm×4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-0.1% phosphoric acid solution with gradient elution. The detection wavelengths were 320 nm in 0-16 min (determination of polygalaxanthone Ⅲ and 3,6'-disinapoyl sucrose), 210 nm in 16-24 min (determination of tenuifolin and onjisaponin B), and 250 nm in 24-35 min (determination of schisandrin). The flow rate was 1.0 mL/min, and the column temperature was set at 30℃ with injection volume of 10 μL. Results There were good linear relationships of polygalaxanthone Ⅲ, 3,6'-disinapoyl sucrose, tenuifolin, onjisaponin B, and schisandrin in the concentration ranges of 3.85-77.00, 6.22-124.40, 6.57-131.40, 7.61-152.20, 4.96-99.20 μg/mL(r≥0.999 2). The average recoveries were 98.17%, 99.24%, 99.90%, 97.63%, and 96.83% with RSD 1.14%, 1.23%, 0.70%, 1.52%, and 0.81%, respectively. Conclusion The method is simple and valid, which can be used in quantity control for Shenrong Anshen Tablets.
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