目的 建立HPLC法测定芪贞慢咽宁颗粒中木蝴蝶苷B。方法 采用Kromasil 100-5 C₁₈色谱柱（250 mm×4.6 mm，5 μm），流动相甲醇-0.1%磷酸（38：62），检测波长276 nm，体积流量1.0 mL/min，柱温30℃，进样量5 μL。结果 木蝴蝶苷B在0.064 6～1.292 0 μg与峰面积线性关系良好。木蝴蝶苷B平均回收率为100.67%，RSD值为1.12%。结论 本法测定快速、结果准确、重复性好、专属性强，可用于测定芪贞慢咽宁颗粒中木蝴蝶苷B。

Objective To establish an HPLC method to determine oroxin B in Qizhen Manyanning Granules. Methods The determination was carried out on Kromasil 100-5 C₁₈ column (250 mm×4.6 mm, 5 μm). The mobile phase consisted of methanol-0.1% phosphoric acid (38:62), and UV detection wavelength was set at 276 nm. The flow rate was 1.0 mL/min and the column temperature was 30℃ with the injection volume of 5 μL. Results There was a good linear relationship between peak area and the content of oroxin B in the rang of 0.064 6-1.292 0 μg. The average recovery of oroxin B was 100.67% with RSD 1.12%. Conclusion The method is rapid, accurate and reproducible for the quantification of oroxin B in Qizhen Manyanning Granules.